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  1. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
  2. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
  3. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 4, December 2016
  4. An Improved Micropropagation and Assessment of Genetic Fidelity in Multipurpose Medicinal Tree, Acacia auriculiformis
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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 4, December 2016
Microglia in the Physiology and Pathology of Brain
Ocimum kilimandscharicum Guerke (Lamiaceae): A New Distributional Record for Peninsular India with Focus on its Economic Potential
Microbial Decolorization and Degradation of Reactive Red 198 Azo Dye by a Newly Isolated Alkaligenes Species
Evaluation of Phytochemicals, Physico-chemical Properties and Antioxidant Activity in Gum Exudates of Buchanania lanzan
Characterization of Vermicomposts Prepared from Agricultural Solid Wastes in North Eastern Hill Region of Nagaland, India
Pedosphere Degradation due to Seasonal Water Logging in Southwestern Punjab
Physiological Growth Indices of Maize Under Semi-Arid Irrigated Conditions as Influenced by Subsoil Compaction and Nitrogen Fertilization
Factors Affecting Recruitment of a Critically-Endangered Dipterocarp Species, Vateria indica in the Western Ghats, India
In Vitro Antibacterial Activity of Ethno Medicinally Used Lichens Against Three Wound Infecting Genera of Enterobacteriaceae
Effect of Packaging Materials and Storage Temperature on Quality of Osmo-vac Dehydrated Guava Slices During Storage
Antioxidant Levels and Activities of Reactive Oxygen-Scavenging Enzymes in Crab Apple Fruits (Malus baccata)
Hydrogen Sulfide Promotes Wheat Grain Germination Under Cadmium Stress
First Attempt of Complete Rearing of Tea Looper, Biston (=Buzura) suppressaria, on Artificial and Natural Diet
Assessment of Antibacterial, Antifungal Property and Purification of Bioactive Compounds from Seagrass, Thalassia hemprichii
Protein Enriched Multigrain Expanded Snack: Optimization of Extrusion Variables
An Improved Micropropagation and Assessment of Genetic Fidelity in Multipurpose Medicinal Tree, Acacia auriculiformis
Moss Flora of Kedarnath Wildlife Sanctuary (KWLS), Garhwal Himalaya, India
Chromosomal Localization and Contribution of Three Homoeologous Genes to Biosynthesis of Cytosolic Aspartate Aminotransferase in Common Wheat
Evaluation of the Murine Dermal Matrix as a Biological Mesh in Dogs
Microbial and Enzyme Activities and Carbon Stock in Unique Coastal Acid Saline Soils of Goa
Relative Efficiency of Zinc Sulphate and Chelated Zinc on Zinc Biofortification of Rice Grains and Zinc Use-Efficiency in Basmati Rice
Morphological and Molecular Characterization of Ectomycorrhizal Amanita Species Associated with Pinus wallichiana A. B. Jacks.
Colonization Factor Antigen I, an Enterotoxigenic Escherichia coli Colonization Factor, Exhibits Therapeutic Significance
Pathogenicity of Different Isolates of Entomopathogenic Fungus, Nomuraea rileyi (Farlow) Samson Against Tobacco Caterpillar, Spodoptera litura (Fabricius)
E-Survey to Analyse Perception of Agricultural Experts Regarding Priority Setting for Future Research in Vegetables in India
A Remarkable Taxon Jackiella javanica Schiffner Variety cordifolia Schiffner Proclaims its First Occurrence in North-eastern India
Structural Insights into the Mode of Action of Plant Flavonoids as Anti-oxidants Using Regression Analysis
Release of Metals from Sludge Amended Acid and Alkaline Soils Under Different Levels of Moisture and Temperature
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 3, September 2016
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 2, June 2016
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 1, March 2016
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 82

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An Improved Micropropagation and Assessment of Genetic Fidelity in Multipurpose Medicinal Tree, Acacia auriculiformis

Content Provider SpringerLink
Author Yadav, Rakesh Kumar, Sandeep Yadav, Neha
Copyright Year 2015
Abstract An efficient direct plant regeneration protocol has been developed for medicinal tree, Acacia auriculiformis that can be employed at a commercial scale. Bioactive properties of this tree attribute to spermicidal action and anti-HIV activity which has gained an increasing interest in world health sector. Among several media employed for present study, an utmost shoot regeneration frequency of 93 % with highest number 17.3 ± 0.61 of shoots per explant was obtained on MS medium supplemented with 2.0 mg l$^{−1}$ KIN and 0.5 mg l$^{−1}$ IAA. Inclusion of activated charcoal in media has effectively alleviated the browning problem of shoots. Maximum shoot length of 6.4 cm was attained on MS media supplemented with 0.25 mg l$^{−1}$ GA$_{3}$ followed by rooting of individual microshoots on half strength MS media supplemented with 0.1 mg l$^{−1}$ IAA. After successful hardening, rooted microshoots were maintained under greenhouse conditions. Genetic stability of micropropagated plants of A. auriculiformis have also been established via randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. A total of 83 (39 RAPD and 44 ISSR) clear, distinct and reproducible amplicons were produced. The banding patterns and similarity coefficients obtained by both RAPD and ISSR analysis have revealed no remarkable differences between tissue culture raised plants and the mother plant. This is the first report that evaluates the use of genetic markers to establish genetic fidelity of micropropagated A. auriculiformis. This method can be applied for the rapid clonal multiplication and true-to-type production of plant for attaining the ever increasing demand in pharmaceutical industries.
Starting Page 921
Ending Page 929
Page Count 9
File Format PDF
ISSN 03698211
Journal Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number 86
Issue Number 4
e-ISSN 22501746
Language English
Publisher Springer India
Publisher Date 2015-05-24
Publisher Place New Delhi
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Life Sciences Plant Biochemistry Nucleic Acid Chemistry RAPD Behavioral Sciences ISSR Medicinal tree Genetic fidelity Acacia auriculiformis
Content Type Text
Resource Type Article
Subject Environmental Science Agricultural and Biological Sciences
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