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  1. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
  2. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
  3. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 4, December 2016
  4. Antioxidant Levels and Activities of Reactive Oxygen-Scavenging Enzymes in Crab Apple Fruits (Malus baccata)
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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 4, December 2016
Microglia in the Physiology and Pathology of Brain
Ocimum kilimandscharicum Guerke (Lamiaceae): A New Distributional Record for Peninsular India with Focus on its Economic Potential
Microbial Decolorization and Degradation of Reactive Red 198 Azo Dye by a Newly Isolated Alkaligenes Species
Evaluation of Phytochemicals, Physico-chemical Properties and Antioxidant Activity in Gum Exudates of Buchanania lanzan
Characterization of Vermicomposts Prepared from Agricultural Solid Wastes in North Eastern Hill Region of Nagaland, India
Pedosphere Degradation due to Seasonal Water Logging in Southwestern Punjab
Physiological Growth Indices of Maize Under Semi-Arid Irrigated Conditions as Influenced by Subsoil Compaction and Nitrogen Fertilization
Factors Affecting Recruitment of a Critically-Endangered Dipterocarp Species, Vateria indica in the Western Ghats, India
In Vitro Antibacterial Activity of Ethno Medicinally Used Lichens Against Three Wound Infecting Genera of Enterobacteriaceae
Effect of Packaging Materials and Storage Temperature on Quality of Osmo-vac Dehydrated Guava Slices During Storage
Antioxidant Levels and Activities of Reactive Oxygen-Scavenging Enzymes in Crab Apple Fruits (Malus baccata)
Hydrogen Sulfide Promotes Wheat Grain Germination Under Cadmium Stress
First Attempt of Complete Rearing of Tea Looper, Biston (=Buzura) suppressaria, on Artificial and Natural Diet
Assessment of Antibacterial, Antifungal Property and Purification of Bioactive Compounds from Seagrass, Thalassia hemprichii
Protein Enriched Multigrain Expanded Snack: Optimization of Extrusion Variables
An Improved Micropropagation and Assessment of Genetic Fidelity in Multipurpose Medicinal Tree, Acacia auriculiformis
Moss Flora of Kedarnath Wildlife Sanctuary (KWLS), Garhwal Himalaya, India
Chromosomal Localization and Contribution of Three Homoeologous Genes to Biosynthesis of Cytosolic Aspartate Aminotransferase in Common Wheat
Evaluation of the Murine Dermal Matrix as a Biological Mesh in Dogs
Microbial and Enzyme Activities and Carbon Stock in Unique Coastal Acid Saline Soils of Goa
Relative Efficiency of Zinc Sulphate and Chelated Zinc on Zinc Biofortification of Rice Grains and Zinc Use-Efficiency in Basmati Rice
Morphological and Molecular Characterization of Ectomycorrhizal Amanita Species Associated with Pinus wallichiana A. B. Jacks.
Colonization Factor Antigen I, an Enterotoxigenic Escherichia coli Colonization Factor, Exhibits Therapeutic Significance
Pathogenicity of Different Isolates of Entomopathogenic Fungus, Nomuraea rileyi (Farlow) Samson Against Tobacco Caterpillar, Spodoptera litura (Fabricius)
E-Survey to Analyse Perception of Agricultural Experts Regarding Priority Setting for Future Research in Vegetables in India
A Remarkable Taxon Jackiella javanica Schiffner Variety cordifolia Schiffner Proclaims its First Occurrence in North-eastern India
Structural Insights into the Mode of Action of Plant Flavonoids as Anti-oxidants Using Regression Analysis
Release of Metals from Sludge Amended Acid and Alkaline Soils Under Different Levels of Moisture and Temperature
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 3, September 2016
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 2, June 2016
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 1, March 2016
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 82

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STUDIES ON ANTIOXIDANT ACTIVITY IN FRUITS OF CRAB APPLE

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Antioxidant Levels and Activities of Reactive Oxygen-Scavenging Enzymes in Crab Apple Fruits (Malus baccata)

Content Provider SpringerLink
Author Sharma, Ruchika Nath, Amarjit K
Copyright Year 2015
Abstract Crab apple (Malus baccata) fruits show higher antioxidant activity, phenols and activities of antioxidant enzymes namely, ascorbate oxidase, catalase, peroxidase, superoxide dismutase except ascorbic acid content as compared to ‘Red Delicious’. Superoxide dismutase was purified and characterized from pulp extract of crab apple fruits by ammonium sulphate precipitation, gel filtration and ion exchange chromatography. Three isoforms of superoxide dismutase were eluted with water, 0.1 molar (M) and 0.2 M potassium chloride (KCl) on anion exchanger. The molecular weight of purified superoxide dismutase isoforms were 15, 17 and 20 kilodaltons (kDa), respectively. Superoxide dismutase isoforms were heat stable up to 90 °C and had pH optima of 7.8. The activity of all the three isoforms increased with increase in concentrations of zinc (Zn$^{2+}$) ions in the reaction mixture. Keeping in view the importance of superoxide dismutase enzyme as a free radical scavenger and its role in stress tolerance in plants, there is a need to identify novel genes of this enzyme from native germplasm.
Starting Page 877
Ending Page 885
Page Count 9
File Format PDF
ISSN 03698211
Journal Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number 86
Issue Number 4
e-ISSN 22501746
Language English
Publisher Springer India
Publisher Date 2015-05-16
Publisher Place New Delhi
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Life Sciences Plant Biochemistry Purification Nucleic Acid Chemistry Antioxidant activity Behavioral Sciences Crab apple Superoxide dismutase
Content Type Text
Resource Type Article
Subject Environmental Science Agricultural and Biological Sciences
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