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  1. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
  2. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
  3. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 4, December 2016
  4. Evaluation of the Murine Dermal Matrix as a Biological Mesh in Dogs
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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 4, December 2016
Microglia in the Physiology and Pathology of Brain
Ocimum kilimandscharicum Guerke (Lamiaceae): A New Distributional Record for Peninsular India with Focus on its Economic Potential
Microbial Decolorization and Degradation of Reactive Red 198 Azo Dye by a Newly Isolated Alkaligenes Species
Evaluation of Phytochemicals, Physico-chemical Properties and Antioxidant Activity in Gum Exudates of Buchanania lanzan
Characterization of Vermicomposts Prepared from Agricultural Solid Wastes in North Eastern Hill Region of Nagaland, India
Pedosphere Degradation due to Seasonal Water Logging in Southwestern Punjab
Physiological Growth Indices of Maize Under Semi-Arid Irrigated Conditions as Influenced by Subsoil Compaction and Nitrogen Fertilization
Factors Affecting Recruitment of a Critically-Endangered Dipterocarp Species, Vateria indica in the Western Ghats, India
In Vitro Antibacterial Activity of Ethno Medicinally Used Lichens Against Three Wound Infecting Genera of Enterobacteriaceae
Effect of Packaging Materials and Storage Temperature on Quality of Osmo-vac Dehydrated Guava Slices During Storage
Antioxidant Levels and Activities of Reactive Oxygen-Scavenging Enzymes in Crab Apple Fruits (Malus baccata)
Hydrogen Sulfide Promotes Wheat Grain Germination Under Cadmium Stress
First Attempt of Complete Rearing of Tea Looper, Biston (=Buzura) suppressaria, on Artificial and Natural Diet
Assessment of Antibacterial, Antifungal Property and Purification of Bioactive Compounds from Seagrass, Thalassia hemprichii
Protein Enriched Multigrain Expanded Snack: Optimization of Extrusion Variables
An Improved Micropropagation and Assessment of Genetic Fidelity in Multipurpose Medicinal Tree, Acacia auriculiformis
Moss Flora of Kedarnath Wildlife Sanctuary (KWLS), Garhwal Himalaya, India
Chromosomal Localization and Contribution of Three Homoeologous Genes to Biosynthesis of Cytosolic Aspartate Aminotransferase in Common Wheat
Evaluation of the Murine Dermal Matrix as a Biological Mesh in Dogs
Microbial and Enzyme Activities and Carbon Stock in Unique Coastal Acid Saline Soils of Goa
Relative Efficiency of Zinc Sulphate and Chelated Zinc on Zinc Biofortification of Rice Grains and Zinc Use-Efficiency in Basmati Rice
Morphological and Molecular Characterization of Ectomycorrhizal Amanita Species Associated with Pinus wallichiana A. B. Jacks.
Colonization Factor Antigen I, an Enterotoxigenic Escherichia coli Colonization Factor, Exhibits Therapeutic Significance
Pathogenicity of Different Isolates of Entomopathogenic Fungus, Nomuraea rileyi (Farlow) Samson Against Tobacco Caterpillar, Spodoptera litura (Fabricius)
E-Survey to Analyse Perception of Agricultural Experts Regarding Priority Setting for Future Research in Vegetables in India
A Remarkable Taxon Jackiella javanica Schiffner Variety cordifolia Schiffner Proclaims its First Occurrence in North-eastern India
Structural Insights into the Mode of Action of Plant Flavonoids as Anti-oxidants Using Regression Analysis
Release of Metals from Sludge Amended Acid and Alkaline Soils Under Different Levels of Moisture and Temperature
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 3, September 2016
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 2, June 2016
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 1, March 2016
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 82

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Evaluation of the Murine Dermal Matrix as a Biological Mesh in Dogs

Content Provider SpringerLink
Author Gangwar, Anil K. Kumar, Vineet Kumar, Naveen
Copyright Year 2015
Abstract In the search of biologically functional biomaterial, murine dermal matrix (MDM) was developed and clinically evaluated as a biological mesh in dogs. Fresh skins of murine origin were de-epithelialized and further decellularized with different detergents over a range of periods. Decellularization completeness was confirmed qualitatively and histologically with hematoxylin and eosin as well as a quantitative Pico-Green assay. Under anesthesia, inguinal and perineal defects were repaired with MDM in ten dogs having body weight of 16.81 ± 3.88 kg and hernial ring size of 21.66 ± 1.60 cm$^{2}$. Serum samples harvested on post-implantation days (0, 15 and 30) were subjected to electrophoretic and immunological analyses. Complete de-epithelialization was achieved with hypertonic saline solution at 8 h. Histologically the matrix treated with 0.5 % sodium dodecyl sulphate (SDS)/0.25 % tri (n-butyl) phosphate (TNBP) for 48 h demonstrated complete acellularity and orderly arranged collagen fibers. Pico-Green assay showed an approximate 93.93 % reduction in double-stranded DNA content for MDM. Defects repaired with MDM healed completely without graft rejection. SDS-PAGE analysis revealed a changed serum protein distribution pattern on post-implantation day 15 as that of day 0 and became normal on post-implantation day 30. Enzyme linked immunosorbent assay confirmed a significantly (P < 0.05) increased antibody titer on day 15 as compared to day 0, thereafter it decreased on day 30 as that of day 15 but still remained significantly (P < 0.05) higher than day 0. These results suggest that 0.5 % SDS/0.25 % TNBP combination for 48 h can be used for complete decellularization of rat skin and developed MDM may be safely used as a biological mesh in dogs.
Starting Page 953
Ending Page 960
Page Count 8
File Format PDF
ISSN 03698211
Journal Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number 86
Issue Number 4
e-ISSN 22501746
Language English
Publisher Springer India
Publisher Date 2015-05-27
Publisher Place New Delhi
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Life Sciences Plant Biochemistry Nucleic Acid Chemistry Enzyme linked immunosorbent assay Inguinal and perineal hernias Behavioral Sciences Decellularization Dogs Murine dermal matrix Sodium dodecyl sulphate polyacrylamide gel electrophoresis
Content Type Text
Resource Type Article
Subject Environmental Science Agricultural and Biological Sciences
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