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  1. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
  2. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
  3. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 3, September 2016
  4. In Vitro Callus Induction and Plantlet Regeneration of Saussurea lappa (Clarke.) from Ladakh Region of India
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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 4, December 2016
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 3, September 2016
Polyploidy in Angiosperms: Genetic Insight to the Phenomenon
Impacts of Rhodococcus equi on Acquired Immunodeficiency Syndrome: A Review
An Impact Assessment of Vegetable Cowpea, Vigna unguiculata (L.) Walp. Variety “Kashi Kanchan”
Combined Effect of Heat Shock and Chlorine Fails to Elicit Acquired Thermal Tolerance in Labeo rohita Spawns
Production of Indole-3-Acetic Acid by Three Species of Seimatosporium Causing Stem Canker and Dieback of Eucalyptus tereticornis
Productivity, Water Use and Energy Profitability of Staggered Maize–Legume Intercropping in the Eastern Himalayan Region of India
Assessment of Age of Seedling and Weed Management Practices on Rice Yield Under System of Rice Intensification
Isolation and Screening of Keratinolytic Bacteria from Feather Dumping Soil in and Around Cuddalore and Villupuram, Tamil Nadu
In Vitro Shoot Multiplication and Root Induction in Silk Banana Variety Nanjanagud Rasabale as Influenced by Monochromatic Light Spectra
Risk Assessment of Some Selected Vegetables Grown in Metal Contaminated Soil Supplements
Effects of Resource Conservation Practices on Productivity, Profitability and Energy Budgeting in Maize–Wheat Cropping System of Indian Sub-Himalayas
Propagation Through Air Layering in Zanthoxylum armatum DC: An Endangered Medicinal Plant in the Himalayan Region
Expression Profile of Leptin and Aromatase-P450 Genes and Association with Sperm Motility in Fresh Bovine Ejaculates
Virulence of Entomopathogenic Fungus, Metarhizium anisopliae to Sweetpotato Whitefly, Bemisia tabaci (Hemiptera: Aleyrodidae) Under Osmotic Stress
Response of Datura innoxia Linn. to Gamma Rays and Its Impact on Plant Growth and Productivity
Stachys gaziantepensis (Lamiaceae), a New Species from South Anatolia, Turkey
Evaluation of Ascorbic Acid and Sodium Metabisulfite Applications for Improvement in Raisin Quality
Evaluation of Cultivated and Wild Allium Accessions for Resistance to Fusarium oxysporum f. sp. cepae
In Vitro Callus Induction and Plantlet Regeneration of Saussurea lappa (Clarke.) from Ladakh Region of India
Alleviation of Cadmium and Mercury Stress by Supplementation of Steroid Hormone to Raphanus sativus Seedlings
How Do Climatic Predictors Affect Tree Richness in Western Ghats, India?
Preliminary Investigations on Identification and Evaluation of Early and Dwarf Lines of Ney Poovan Banana (Musa AB) for Commercial Scale Utilization
Extracellular Laccase from a Newly Isolated Psychrotolerant Strain of Cladosporium tenuissimum (NFCCI 2608)
Soil Nutrient Status and Leaf Nutrient Norms in Oil Palm (Elaeis guineensis Jacq.) Plantations Grown on Southern Plateau of India
Efficacy of Food Preservatives and Volatile Compounds in the Control of Ochratoxin A Production by Penicillium Species
Characterization and Mosquitocidal Potential of the Soil Bacteria Aneurinibacillus aneurinilyticus Isolated from Burdwan, West Bengal, India
Effect of Host Plants on Insecticide Susceptibility and Detoxification Enzymes Activity in Spodoptera litura Fabricius (Noctuidae: Lepidoptera)
Rhizobium Mutant Deficient in Mineral Phosphate Solubilization Activity Shows Reduced Nodulation and Plant Growth in Green Gram
Substratum as Determining Factor for the Distribution of Benthic Macroinvertebrate Fauna in a River Ecosystem
Bio-efficacy of Herbicides on Performance of Mustard, Brassica juncea (L.) and Population Dynamics of Agriculturally Important Bacteria
In Vitro Activity and the Efficacy of Arbekacin, Cefminox, Fosfomycin, Biapenem Against Gram-Negative Organisms: New Treatment Options?
Ethiopian Mustard–Chickpea Intercropping System is a Viable Option for Yield Advantage in Dryland Condition of North India-Part II
Comparative Study of Physical Factors and Microbial Diversity of Four Man-Made Extreme Ecosystems
Erratum to: Total Polyphenols, Antioxidant and Antimicrobial Activity of Leaves and Stem Bark Extracts of Allophylus cobbe (L.) Raeusch
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 2, June 2016
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86, Issue 1, March 2016
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 82

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In Vitro Callus Induction and Plantlet Regeneration of Saussurea lappa (Clarke.) from Ladakh Region of India

Content Provider SpringerLink
Author Kumar, Jitendra Chaurasia, Om P. Kapoor, Sahil Bajpai, Prabodh K. Rewang, Stanzin Srivastava, Ravi B. Warghat, Ashish R.
Copyright Year 2015
Abstract In vitro propagation protocol was developed for Saussurea lappa (Clarke.) species threatened by over exploitation due to medicinal importance and habitat destruction in Ladakh region of India. The aim of the present study was to examine the main aspects of in vitro callus induction (CI) and plantlet regeneration of S. lappa. Explants were cultured on Murashige and Skoog (MS) basal medium supplemented with various concentrations and combinations of auxins and cytokinins for in vitro CI and plantlets regeneration. Callus initiation and induction was observed within eight and fifteen days after inoculation. 3 mg/L 2, 4-Dichlorophenoxyacetic acid (2, 4-D) and 5 mg/L Kinetin (Kin) was found to be more effective for CI frequencies in all the explants as compared to other combinations. Root (79.6 ± 0.8) and stem (76.4 ± 0.6) explants were found with better callus frequencies and early response as compared to other explants. Maximum number of shoots (11.8 ± 0.7), highest shoot length (9.2 ± 0.6 cm), maximum root number (6.2 ± 0.7) and highest root length (6.6 ± 0.8 cm) were found on MS medium with 2 mg/L Kinetin (Kin) and 2 mg/L Indole-3-butyric-acid in root derived callus explant. Plantlets with 3–5 shoots were transferred to potting mixture containing sand: soil: perlite (1:1:1) for acclimatization to field conditions and further multiplication. For the first time, protocol has been developed in S. lappa for in vitro CI and plantlet regeneration that holds robust potential for metabolite production and large-scale propagation.
Starting Page 651
Ending Page 660
Page Count 10
File Format PDF
ISSN 03698211
Journal Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number 86
Issue Number 3
e-ISSN 22501746
Language English
Publisher Springer India
Publisher Date 2015-03-19
Publisher Place New Delhi
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Life Sciences Growth regulator Plant Biochemistry Nucleic Acid Chemistry Behavioral Sciences Callus induction Plantlet regeneration Acclimatization
Content Type Text
Resource Type Article
Subject Environmental Science Agricultural and Biological Sciences
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