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  1. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
  2. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
  3. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 3, September 2017
  4. Enhanced In Vitro Shoot Regeneration in Oldenlandia umbellata L. by Using Quercetin: A Naturally Occurring Auxin-Transport Inhibitor
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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 4, December 2017
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 3, September 2017
Microbial Fuel Cells and Their Applications for Cost Effective Water Pollution Remediation
Development of Quick Cooking Rice: Application of Salts and Enzyme Pretreatments
Molecular Identification of Dominant Cultivars of Soybean Using Simple Sequence Repeat Markers
Altered Expression Pattern of Major Seminal Plasma Protein Genes in Seminal Vesicles of Water Buffalo (Bubalus bubalis)
Feeding Deterrence Effects of Defatted Jojoba (Simmondisa chinensis) Meal Against Indian Gerbil, Tatera indica (Hardwicke)
Yield and Quality of Isabgol (Plantago ovata Forsk) as Influenced by Micronutrient Application in Soil
Comparison of Pharmacokinetics of Enrofloxacin After Single Oral Bolus and Pulse Dose Administration in Broiler Chicken
In silico characterization, homology modeling of Camellia sinensis chitinase and its evolutionary analyses with other plant chitinases
Effect of Zinc Sulphate and Organics on Zn Content and Yield of Chenopodium Grown in Different Soils : Agronomic Bio-fortification of Chenopodium with Zn
Effect of Diffusion Channel Storage on Some Physical Properties of Button Mushroom (Agaricus bisporus) and Shelf-Life Extension
Identification and Expression Analysis of Thyroid Stimulating Hormone Receptor (TSHR) in Fish Gonads Following LHRH Treatment
Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides
Eggshells of Drosophila melanogaster and D. simulans: Ultrastructure, Measurement and Analyses
Cytomorphological Analyses of Abnormal Blood Cells of Acute Leukemic Patients
Dissipation and Safety Evaluation of Tebuconazole Residues in Peanut-Field Ecosystem
Bt Cotton–Groundnut Intercropping System: A Pragmatic Approach for Increasing Edible Oilseeds Production in India
Nutritional and Antioxidant Potential of Aegle marmelos Fermented Fruit Juice
Impact Assessment on Floral Composition and Spread Potential of Mikania micrantha H.B.K. in an Urban Scenario
Antagonistic Actinomycetes Mediated Resistance in Solanum lycopersicon Mill. Against Rhizoctonia solani Kühn
Use of Piriformospora indica as Potential Biological Hardening Agent for Endangered Micropropagated Picrorhiza kurroa Royel ex Benth
Investigation on Optimally Performing Sensor Substrates through Bio-fouling of Immunoglobulin-Conjugated Gold Nanoparticles
Genetic Fitness and Bottleneck of Threadfin (Sea Catfish), Arius arius (Hamilton, 1822) in South Indian Coastline
Molecular Characterization of Differentially Expressed ADP Ribosylation Factor from WSSV Infected Black Tiger Shrimp Penaeus monodon
Biodegradation of Isoproturon by Bacillus pumilus K1 Isolated from Foothill Agroecosystem of North West Himalaya
Improvisation of Pressmud by Organic Amendment and Its Effect on Soil Quality and Growth of Chilli, Capsicum annum
Microsatellite-Based DNA Fingerprinting of Guava (Psidium guajava) Genotypes
Surface Drainage in Transplanted Rice: Productivity, Relative Water and Leaf Rolling, Root Behaviour and Weed Dynamics
Combining Ability Analysis and Its Relationship with Gene Action and Heterosis in Early Maturity Cauliflower
Protein Sparing Effect of Dietary Rumen Protected Lysine Plus Methionine in Growing Murrah Buffaloes (Bubalus bubalis)
Leopard–Human Conflict Led Casualties and Conservation Awareness Campaign in Shivalik Hills of Northern India
Enhanced In Vitro Shoot Regeneration in Oldenlandia umbellata L. by Using Quercetin: A Naturally Occurring Auxin-Transport Inhibitor
Antibiotic Potential of Endophytic Actinomycetes of Medicinal Herbs Against Human Pathogenic Bacteria
Genetic Diversity in Corchorus olitorius Genotypes Using Jute SSRs
Effect of Sulphur and Cadmium on Macronutrient Balance in Spring Wheat
A Novel Approach to Evaluate Titanium Dioxide Nanoparticle–Protein Interaction Through Docking: An Insight into Mechanism of Action
Molecular Cloning, Domain Analysis and Homology Modeling of Toll-Like Receptor 3 from Barilius bendelisis (Ham.)
Submergence Induced Tiller Mortality and Yield Reduction in Rice Can be Minimized Through Post-submergence Nitrogen Application
Effect of Nitrogen and Shade on Photosynthetic Gas Exchange and Productivity of Wheat Under Elevated Atmospheric CO$_{2}$ Concentration
Biochar and Crop Residue Amendments on Soil Microbial and Biochemical Properties
Enzymatic Efficiency and Genotypic Differences for Nitrogen Assimilation in Wheat
Evaluation of Antitumor Activity of Glutaminase-Free Periplasmic Asparaginase from Indigenous Bacterial Isolates as Candidates for Cancer Therapy
Haplotypic Diversity and Virulence of Xanthomonas oryzae pv. oryzae Population Infecting the First Superfine Aromatic Basmati Hybrid
Isolation, Screening and Evaluation of Antifouling Activity of Mangrove Associated Bacterium, Bacillus subtilis subsp. subtilis RG
Studies on Tribe Onitini (Scarabaeinae: Scarabaeidae: Coleoptera) from Madhya Pradesh with a Checklist from India
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 2, June 2017
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 1, March 2017
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 82

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Enhanced In Vitro Shoot Regeneration in Oldenlandia umbellata L. by Using Quercetin: A Naturally Occurring Auxin-Transport Inhibitor

Content Provider SpringerLink
Author Saranya Krishnan, S. R. Siril, E. A.
Copyright Year 2015
Abstract An efficient in vitro propagation method for a dye yielding medicinal herb, Oldenlandia umbellata L. was established using benzyl adenine and quercetin. Nodal segments cultured on agar gelled Murashige and Skoog medium supplemented with benzyl adenine (5.0 μM) and quercetin (5 μM), produced maximum explant response (100 %) and number of shoots (118.67). Thus a continuous shoot multiplication system in every 4 weeks interval was established. Addition of quercetin in combination with benzyl adenine produced significantly (p < 0.001) higher number of shoots than medium supplemented benzyl adenine alone and in short, addition of quercetin resulted in 1.5 fold increase in shoot number. Addition of 5 µM quercetin in combination with 5 µM benzyl adenine resulted in the production of 4.64 cm sized shoots with an average of 4.13 nodes per shoot. In vitro raised microshoots were rooted ex vitro, by a pulse treatment in 50 µM indole-3-butyric acid for 30 s, followed by planting in plastic cups containing potting mixture. Ex vitro planted microshoots were rooted and recorded high rate of survival (81.3 %). The present protocol in brief, suggests that by modifying auxin transport in a tissue culture system, rate of shoot multiplication can be increased, thus facilitating mass propagation of plants having strong apical dominance.
Starting Page 899
Ending Page 904
Page Count 6
File Format PDF
ISSN 03698211
Journal Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number 87
Issue Number 3
e-ISSN 22501746
Language English
Publisher Springer India
Publisher Date 2015-10-26
Publisher Place New Delhi
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Oldenlandia umbellata Life Sciences Auxin transport inhibitor Plant Biochemistry Nucleic Acid Chemistry Benzyl adenine Behavioral Sciences Nodal explants Quercetin In vitro multiplication
Content Type Text
Resource Type Article
Subject Environmental Science Agricultural and Biological Sciences
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