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  1. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
  2. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
  3. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 3, September 2017
  4. Altered Expression Pattern of Major Seminal Plasma Protein Genes in Seminal Vesicles of Water Buffalo (Bubalus bubalis)
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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 4, December 2017
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 3, September 2017
Microbial Fuel Cells and Their Applications for Cost Effective Water Pollution Remediation
Development of Quick Cooking Rice: Application of Salts and Enzyme Pretreatments
Molecular Identification of Dominant Cultivars of Soybean Using Simple Sequence Repeat Markers
Altered Expression Pattern of Major Seminal Plasma Protein Genes in Seminal Vesicles of Water Buffalo (Bubalus bubalis)
Feeding Deterrence Effects of Defatted Jojoba (Simmondisa chinensis) Meal Against Indian Gerbil, Tatera indica (Hardwicke)
Yield and Quality of Isabgol (Plantago ovata Forsk) as Influenced by Micronutrient Application in Soil
Comparison of Pharmacokinetics of Enrofloxacin After Single Oral Bolus and Pulse Dose Administration in Broiler Chicken
In silico characterization, homology modeling of Camellia sinensis chitinase and its evolutionary analyses with other plant chitinases
Effect of Zinc Sulphate and Organics on Zn Content and Yield of Chenopodium Grown in Different Soils : Agronomic Bio-fortification of Chenopodium with Zn
Effect of Diffusion Channel Storage on Some Physical Properties of Button Mushroom (Agaricus bisporus) and Shelf-Life Extension
Identification and Expression Analysis of Thyroid Stimulating Hormone Receptor (TSHR) in Fish Gonads Following LHRH Treatment
Isolation and Characterization of Novel Polymorphic Microsatellite Loci in Hippophae rhamnoides
Eggshells of Drosophila melanogaster and D. simulans: Ultrastructure, Measurement and Analyses
Cytomorphological Analyses of Abnormal Blood Cells of Acute Leukemic Patients
Dissipation and Safety Evaluation of Tebuconazole Residues in Peanut-Field Ecosystem
Bt Cotton–Groundnut Intercropping System: A Pragmatic Approach for Increasing Edible Oilseeds Production in India
Nutritional and Antioxidant Potential of Aegle marmelos Fermented Fruit Juice
Impact Assessment on Floral Composition and Spread Potential of Mikania micrantha H.B.K. in an Urban Scenario
Antagonistic Actinomycetes Mediated Resistance in Solanum lycopersicon Mill. Against Rhizoctonia solani Kühn
Use of Piriformospora indica as Potential Biological Hardening Agent for Endangered Micropropagated Picrorhiza kurroa Royel ex Benth
Investigation on Optimally Performing Sensor Substrates through Bio-fouling of Immunoglobulin-Conjugated Gold Nanoparticles
Genetic Fitness and Bottleneck of Threadfin (Sea Catfish), Arius arius (Hamilton, 1822) in South Indian Coastline
Molecular Characterization of Differentially Expressed ADP Ribosylation Factor from WSSV Infected Black Tiger Shrimp Penaeus monodon
Biodegradation of Isoproturon by Bacillus pumilus K1 Isolated from Foothill Agroecosystem of North West Himalaya
Improvisation of Pressmud by Organic Amendment and Its Effect on Soil Quality and Growth of Chilli, Capsicum annum
Microsatellite-Based DNA Fingerprinting of Guava (Psidium guajava) Genotypes
Surface Drainage in Transplanted Rice: Productivity, Relative Water and Leaf Rolling, Root Behaviour and Weed Dynamics
Combining Ability Analysis and Its Relationship with Gene Action and Heterosis in Early Maturity Cauliflower
Protein Sparing Effect of Dietary Rumen Protected Lysine Plus Methionine in Growing Murrah Buffaloes (Bubalus bubalis)
Leopard–Human Conflict Led Casualties and Conservation Awareness Campaign in Shivalik Hills of Northern India
Enhanced In Vitro Shoot Regeneration in Oldenlandia umbellata L. by Using Quercetin: A Naturally Occurring Auxin-Transport Inhibitor
Antibiotic Potential of Endophytic Actinomycetes of Medicinal Herbs Against Human Pathogenic Bacteria
Genetic Diversity in Corchorus olitorius Genotypes Using Jute SSRs
Effect of Sulphur and Cadmium on Macronutrient Balance in Spring Wheat
A Novel Approach to Evaluate Titanium Dioxide Nanoparticle–Protein Interaction Through Docking: An Insight into Mechanism of Action
Molecular Cloning, Domain Analysis and Homology Modeling of Toll-Like Receptor 3 from Barilius bendelisis (Ham.)
Submergence Induced Tiller Mortality and Yield Reduction in Rice Can be Minimized Through Post-submergence Nitrogen Application
Effect of Nitrogen and Shade on Photosynthetic Gas Exchange and Productivity of Wheat Under Elevated Atmospheric CO$_{2}$ Concentration
Biochar and Crop Residue Amendments on Soil Microbial and Biochemical Properties
Enzymatic Efficiency and Genotypic Differences for Nitrogen Assimilation in Wheat
Evaluation of Antitumor Activity of Glutaminase-Free Periplasmic Asparaginase from Indigenous Bacterial Isolates as Candidates for Cancer Therapy
Haplotypic Diversity and Virulence of Xanthomonas oryzae pv. oryzae Population Infecting the First Superfine Aromatic Basmati Hybrid
Isolation, Screening and Evaluation of Antifouling Activity of Mangrove Associated Bacterium, Bacillus subtilis subsp. subtilis RG
Studies on Tribe Onitini (Scarabaeinae: Scarabaeidae: Coleoptera) from Madhya Pradesh with a Checklist from India
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 2, June 2017
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 1, March 2017
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 82

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Altered Expression Pattern of Major Seminal Plasma Protein Genes in Seminal Vesicles of Water Buffalo (Bubalus bubalis)

Content Provider SpringerLink
Author Ghosh, S. K. Shende, Ashish Pillai, Harikrishna Mahaver, Manish Bhure, S. K. Sarkar, Mihir
Copyright Year 2015
Abstract The binder of sperm proteins (BSP) are secretary products of seminal vesicles. Secretions from the seminal vesicles contribute more to seminal plasma which determine the quality of the semen. The concentration of BSPs in seminal plasma has been negatively correlated with cryopreservation. An analysis of expression profile of seminal plasma proteins could be critical for better freezability of semen. Present study was carried out to assess the expression levels of major seminal plasma protein genes viz., BSP1, BSP3 and BSP5 from seminal vesicles of water buffalo using Real Time PCR. The study consisted of identification of a suitable reference gene in seminal vesicles followed by the expression analysis of major bovine seminal plasma protein (BSP) genes. The most stable reference gene among the three (glyceraldehyde 3-phosphate dehydrogenase, ribosomal protein S15a (RPS15a) and beta-actin), was found to be RPS15a and therefore, was used for normalization of target gene expression. Data analysis of major BSPs showed highest relative expression of BSP3 followed by BSP1 and BSP5. The ratio of relative expression of BSP1:BSP3:BSP5 was 10:18:1. The results from the study also showed an altered expression pattern of major BSP genes in buffalo seminal vesicles. Thus, it can be concluded that the altered expression of these genes at mRNA level would also be reflected at protein level in the seminal plasma. This could be one of the probable reasons for poor freezability of buffalo semen as compared to cattle and the finding helps in designing better cryopreservation strategies for buffalo semen.
Starting Page 655
Ending Page 661
Page Count 7
File Format PDF
ISSN 03698211
Journal Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number 87
Issue Number 3
e-ISSN 22501746
Language English
Publisher Springer India
Publisher Date 2015-08-26
Publisher Place New Delhi
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Life Sciences Plant Biochemistry Nucleic Acid Chemistry Seminal vesicle Behavioral Sciences BSP5 Relative gene expression BSP3 BSP1 Buffalo
Content Type Text
Resource Type Article
Subject Environmental Science Agricultural and Biological Sciences
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