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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Miller, Benjamin L. Striemer, Christopher C. Mace, Charles R. |
Description | Country affiliation: United States Author Affiliation: Mace CR ( Department of Biochemistry and Biophysics, University of Rochester, Rochester, NY 14642, United States.) |
Abstract | Assays built upon protein arrays are critical tools in determining the basic nature of biology, and have considerable promise in diagnosing human disease. These protein arrays aid in the elucidation of mapping pathway interactions, disease biomarker discovery, and regulatory processes. The solutions used in these experiments, including cellular lysate and serum, are inherently complex mixtures and are high in total protein content. Therefore, array-based assays must be robust and maintain a high level of selectivity and sensitivity. We report herein that arrayed imaging reflectometry (AIR), a label-free biosensing platform we have previously disclosed, is highly suitable for the detection of human proteins in complex solutions. In particular, we demonstrate array-based detection of cytokines in buffered solutions, and in undiluted human serum. |
ISSN | 09565663 |
e-ISSN | 18734235 |
DOI | 10.1016/j.bios.2008.05.003 |
Journal | Biosensors and Bioelectronics |
Issue Number | 2 |
Volume Number | 24 |
Language | English |
Publisher | Elsevier |
Publisher Date | 2008-10-15 |
Publisher Place | Great Britain (UK) |
Access Restriction | Open |
Subject Keyword | Biosensing Techniques Instrumentation Photometry Protein Array Analysis Proteins Transducers Equipment Design Equipment Failure Analysis Reproducibility Of Results Sensitivity And Specificity Evaluation Studies Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s. Discipline Biotechnology |
Content Type | Text |
Resource Type | Article |
Subject | Nanoscience and Nanotechnology Biomedical Engineering Biophysics Electrochemistry Biotechnology Medicine |
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