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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Wallenius, Janne Maaheimo, Hannu Eerikäinen, Tero |
Description | Author Affiliation: Wallenius J ( Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, P.O. Box 6100, FIN-02015, Finland. Electronic address: janne.wallenius@aalto.fi.); Maaheimo H ( VTT, Technical Research Centre of Finland Ltd, P.O. Box 1000, FI-02044 VTT Espoo, Finland.); Eerikäinen T ( Aalto University, School of Chemical Technology, Department of Biotechnology and Chemical Technology, P.O. Box 6100, FIN-02015, Finland.) |
Abstract | The metabolism of butanol producing bacteria Clostridium acetobutylicum was studied in chemostat with glucose limited conditions, butanol stimulus, and as a reference cultivation. COnstraint-Based Reconstruction and Analysis (COBRA) was applied using additional constraints from (13)C Metabolic Flux Analysis ((13)C-MFA) and experimental measurement results. A model consisting of 451 metabolites and 604 reactions was utilized in flux balance analysis (FBA). The stringency of the flux spaces considering different optimization objectives, i.e. growth rate maximization, ATP maintenance, and NADH/NADPH formation, for flux variance analysis (FVA) was studied in the different modelled conditions. Also a previously uncharacterized exopolysaccharide (EPS) produced by C. acetobutylicum was characterized on monosaccharide level. The major monosaccharide components of the EPS were 40n-% rhamnose, 34n-% glucose, 13n-% mannose, 10n-% galactose, and 2n-% arabinose. The EPS was studied to have butanol adsorbing property, 70(butanol)mg(EPS)g(-1) at 37°C. |
ISSN | 09608524 |
Journal | Bioresource Technology |
Volume Number | 219 |
e-ISSN | 18732976 |
Language | English |
Publisher | Elsevier |
Publisher Date | 2016-11-01 |
Publisher Place | Great Britain (UK) |
Access Restriction | Subscribed |
Subject Keyword | Discipline Bioresource |
Content Type | Text |
Resource Type | Article |
Subject | Bioengineering Renewable Energy, Sustainability and the Environment Waste Management and Disposal Environmental Engineering Medicine |
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