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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Walter, M. A. Hofker, M. H. Cox, D. W. |
Description | Author Affiliation: Hofker MH ( Research Institute, Hospital For Sick Children, Toronto, ON, Canada.); |
Abstract | We have found by pulsed-field gel electrophoresis that the human immunoglobulin heavy chain constant region gene complex maps entirely to a 350-kilobase (kb) Mlu I fragment. The enzyme Eag I was used with pulsed-field gel electrophoresis alone and in double digests with Spe I to map the region. C gamma 3, of the C gamma 3-C gamma 1-C psi epsilon 1-C alpha 1 cluster, maps 60 kb to the 3' side of C delta; C gamma 2 of the C gamma 2-C gamma 4-C epsilon-C alpha 2 cluster maps 80 kb to the 3' side of C alpha 1, where C gamma 3 encodes the constant region of the immunoglobulin gamma 3 chain, C gamma 1 encodes the constant region of the immunoglobulin gamma 1 chain, etc. C psi gamma maps 35 kb to the 3' side of C alpha 1 and is in the same transcriptional orientation as the other genes. Although in the cloned DNA many CpG-containing restriction sites were identified, most of these were methylated in peripheral blood leukocytes. The sites that were not methylated were predominantly found in three clusters, or Hpa I tiny fragment islands. One was found on the 5' side of C mu; the other two lie 30 kb to the 3' side of each of the C alpha genes and could indicate the presence of regulatory sequences or genes. A region showing strong linkage disequilibrium between all C gamma genes spans at least 160 kb. The 70-kb C mu-C gamma 3 region, however, shows no linkage disequilibrium, possibly indicating a recombination hot spot. The immunoglobulin heavy chain constant region has been almost entirely cloned and mapped, and thus most rearrangements occurring in this region should be detectable. |
ISSN | 00278424 |
e-ISSN | 10916490 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Issue Number | 14 |
Volume Number | 86 |
Language | English |
Publisher | National Academy of Sciences |
Publisher Date | 1989-08-01 |
Publisher Place | United States |
Access Restriction | Open |
Subject Keyword | Genes, Immunoglobulin Immunoglobulin Constant Regions Genetics Immunoglobulin Heavy Chains Blotting, Southern Cloning, Molecular Cosmids DNA Isolation & Purification Electrophoresis Restriction Mapping Research Support, Non-U.S. Gov't Multidisciplinary |
Content Type | Text |
Resource Type | Article |
Subject | Multidisciplinary |
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