NDLI: Selective delipidation of plasma HDL enhances reverse cholesterol transport in vivo.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sacks, Frank M. Rudel, Lawrence L. Conner, Adam Akeefe, Hassibullah Kostner, Gerhard Baki, Talal Rothblat, George de la Llera-Moya, Margarita Asztalos, Bela Perlman, Timothy Zheng, Chunyu Alaupovic, Petar Maltais, Jo-Ann B. Brewer, H. Bryan
Description	Country affiliation: United States Author Affiliation: Sacks FM ( Harvard School of Public Health and Harvard Medical School, Boston, MA, USA, USA. fsacks@hsph.harvard.edu)
Abstract	Uptake of cholesterol from peripheral cells by nascent small HDL circulating in plasma is necessary to prevent atherosclerosis. This process, termed reverse cholesterol transport, produces larger cholesterol-rich HDL that transfers its cholesterol to the liver facilitating excretion. Most HDL in plasma is cholesterol-rich. We demonstrate that treating plasma with a novel selective delipidation procedure converts large to small HDL [HDL-selectively delipidated (HDL-sdl)]. HDL-sdl contains several cholesterol-depleted species resembling small alpha, prebeta-1, and other prebeta forms. Selective delipidation markedly increases efficacy of plasma to stimulate ABCA1-mediated cholesterol transfer from monocytic cells to HDL. Plasma from African Green monkeys underwent selective HDL delipidation. The delipidated plasma was reinfused into five monkeys. Prebeta-1-like HDL had a plasma residence time of 8 +/- 6 h and was converted entirely to large alpha-HDL having residence times of 13-14 h. Small alpha-HDL was converted entirely to large alpha-HDL. These findings suggest that selective HDL delipidation activates reverse cholesterol transport, in vivo and in vitro. Treatment with delipidated plasma tended to reduce diet-induced aortic atherosclerosis in monkeys measured by intravascular ultrasound. These findings link the conversion of small to large HDL, in vivo, to improvement in atherosclerosis.
File Format	HTM / HTML
ISSN	00222275
e-ISSN	15397262
DOI	10.1194/jlr.M800622-JLR200
Journal	The Journal of Lipid Research
Issue Number	5
Volume Number	50
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2009-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Biochemistry Cholesterol Metabolism Lipid Metabolism Lipoproteins, Ldl Atp Binding Cassette Transporter 1 Atp-binding Cassette Transporters Animals Aorta Pathology Apolipoprotein A-i Atherosclerosis Physiopathology Biological Transport Cell Line Cercopithecus Aethiops Lipoproteins, Hdl Blood Chemistry Mice Scavenger Receptors, Class B Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Endocrinology Biochemistry Cell Biology

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