NDLI: Dissecting the kinematics of the kinesin step.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhang, Zhechun Thirumalai, D.
Description	Country affiliation: United States Author Affiliation: Zhang Z ( Biophysics Program, Institute for Physical Science and Technology, University of Maryland, College Park, MD 20742, USA.)
Abstract	Kinesin walks processively on microtubules in an asymmetric hand-over-hand manner with each step spanning 16 nm. We used molecular simulations to determine the fraction of a single step due to conformational changes in the neck linker, and that due to diffusion of the tethered head. Stepping is determined largely by two energy scales, one favoring neck-linker docking and the other, Îµ(h)(MT-TH), between the trailing head (TH) and the microtubule. Neck-linker docking and an optimal value of Îµ(h)(MT-TH) are needed to minimize the probability that the TH takes side steps. There are three major stages in the kinematics of a step. In the first, the neck linker docks, resulting in â¼(5-6) nm movements of the trailing head. The TH moves an additional (6-8) nm in stage II by anisotropic translational diffusion. In the third stage, spanning â¼(3-4) nm, the step is complete with the TH binding to the ß-tubulin binding site.
File Format	HTM / HTML
ISSN	09692126
Issue Number	4
Volume Number	20
e-ISSN	18784186
Journal	Structure
Language	English
Publisher	Elsevier
Publisher Date	2012-04-04
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Discipline Biotechnology Adenosine Triphosphate Chemistry Kinesin Microtubules Molecular Dynamics Simulation Tubulin Binding Sites Biomechanical Phenomena Diffusion Kinetics Protein Binding Protein Structure, Secondary Protein Structure, Tertiary Thermodynamics Journal Article Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject Domain (in MeSH)	Cells Heterocyclic Compounds Macromolecular Substances Enzymes and Coenzymes Investigative Techniques Physical Phenomena Chemical Phenomena
Subject	Structural Biology Molecular Biology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Mr. Siddhartha Mukherjee siddhartha@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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