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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Irvine, Danielle V. Goto, Derek B. Vaughn, Matthew W. Nakaseko, Yukinobu McCombie, W. Richard Yanagida, Mitsuhiro Martienssen, Rob |
Description | Country affiliation: United States Author Affiliation: Irvine DV ( Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA.) |
Abstract | Fission yeast is an important model for epigenetic studies due to the ease with which genetic mutants can be isolated. However, it can be difficult to complement epigenetic phenotypes with genomic libraries in order to identify the genes responsible. This is because epigenetic phenotypes are typically unstable, and can prohibit complementation if silencing cannot be reestablished. Here we have resequenced the fission yeast genome following mutagenesis to readily identify a novel mutant involved in heterochromatic silencing. Candidate genes were identified as functional single base changes linked to the mutation, which were then reconstituted in a wild-type strain to recapitulate the mutant phenotype. By this procedure we identified a weak allele of ubc4, which encodes an essential E2 ubiquitin ligase, as responsible for the swi*603 mutant phenotype. In combination with a large collection of mutants and suppressor plasmids, next-generation genomic resequencing promises to dramatically enhance the power of yeast genetics, permitting the isolation of subtle alleles of essential genes, alleles with quantitative effects, and enhancers and suppressors of heterochromatic silencing. |
File Format | HTM / HTML |
ISSN | 10889051 |
e-ISSN | 15495469 |
DOI | 10.1101/gr.089318.108 |
Journal | Genome Research |
Issue Number | 6 |
Volume Number | 19 |
Language | English |
Publisher | Cold Spring Harbor Laboratory Press |
Publisher Date | 2009-06-01 |
Publisher Place | United States |
Access Restriction | Open |
Subject Keyword | Discipline Genetics Discipline Genomics Genome, Fungal Genetics Mutation Schizosaccharomyces Sequence Analysis, Dna Amino Acid Substitution Chromosome Mapping Chromosomes, Fungal Epigenesis, Genetic Fungal Proteins Genes, Essential Phenotype Polymorphism, Single Nucleotide Ubiquitin-conjugating Enzymes Research Support, N.i.h., Extramural |
Content Type | Text |
Resource Type | Article |
Subject | Genetics (clinical) Genetics |
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