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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Karpagam, R. Preeti, R. Ashokkumar, B. Varalakshmi, P. |
Description | Country affiliation: India Author Affiliation: Karpagam R ( Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.); Preeti R ( Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.); Ashokkumar B ( Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India.); Varalakshmi P ( Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Madurai, Tamil Nadu, India. Electronic address: vara5277@gmail.com.) |
Abstract | Lipid from microalgae is one of the putative oil resources to facilitate the biodiesel production during this era of energy dissipation and environmental pollution. In this study, the key parameters such as biomass productivity, lipid productivity and lipid content were evaluated at the early stationary phase of Chlamydomonas reinhardtii, CC1010 cultivated in nutrient starved (nitrogen, phosphorous), glucose (0.05%, 0.1%, 0.15% and 0.2%) and vitamin B12 supplementation (0.001%, 0.002% and 0.003%) in Tris-Acetate-Phosphate (TAP) medium. The lipid content in nitrogen starved media was 61% which is 2.34 folds higher than nutrient sufficient TAP medium. Glucose supplementation has lead to proportional increase in biomass productivity with the increasing concentration of glucose whereas vitamin B12 supplementations had not shown any influence in lipid and biomass production. Further, fatty acid methyl ester (FAME) profiling of C. reinhardtii, CC 1010 has revealed more than 80% of total SFA (saturated fatty acid) and MUFA (mono unsaturated fatty acid) content. Quality checking parameters of biodiesel like cetane number, saponification value, iodine number and degree of unsaturation were analyzed and the biodiesel fuel properties were found to be appropriate as per the international standards, EN 14214 and ASTM D6751. Conclusively, among all the treatments, nitrogen starvation with 0.1% glucose supplementation had yielded high lipid content in C. reinhardtii, CC 1010. |
File Format | HTM / HTML |
ISSN | 01476513 |
Volume Number | 121 |
e-ISSN | 10902414 |
Journal | Ecotoxicology and Environmental Safety |
Language | English |
Publisher | Elsevier |
Publisher Date | 2015-11-01 |
Publisher Place | Netherlands |
Access Restriction | Subscribed |
Subject Keyword | Discipline Environmental Sciences Biofuels Biotechnology Methods Chlamydomonas Reinhardtii Growth & Development Metabolism Fatty Acids Biosynthesis Standards Biomass Analysis Gas Chromatography-mass Spectrometry Glucose Lipids Microalgae Microscopy, Confocal Nitrogen Phosphorus Journal Article Research Support, Non-u.s. Gov't |
Content Type | Text |
Resource Type | Article |
Subject Domain (in MeSH) | Eukaryota Organism Forms Inorganic Chemicals Carbohydrates Lipids Complex Mixtures Diagnosis Investigative Techniques Biological Phenomena Natural Science Disciplines |
Subject | Health, Toxicology and Mutagenesis Pollution Public Health, Environmental and Occupational Health |
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