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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Cherpelis, Basil S. Turner, Logan Ladd, Sharron Glass, L. Frank Fenske, Neil A. |
Description | Country affiliation: United States Author Affiliation: Cherpelis BS ( Department of Dermatology and Cutaneous Surgery, University of South Florida, Tampa, Florida, USA. bcherpel@health.usf.edu) |
Abstract | BACKGROUND: Dense inflammation can obscure nonmelanoma skin cancer (NMSC) on frozen sections, prompting removal of additional layers to ensure negative margins. Cytokeratin (CK) immunostaining in Mohs micrographic surgery (MMS) has been examined and found to be useful but is limited by lengthy 1-hour processing. OBJECTIVE: Our objective was to develop an effective ultrarapid CK frozen section immunostain to be used during MMS in cases of NMSC with dense or perineural inflammation. METHODS: An ultrarapid immunostain with a mixture of AE1/AE3 monoclonal antibodies was performed in 21 MMS cases and compared with permanent sections prepared from the same material. RESULTS: The ultrarapid CK protocol stained all of the cells in each of the 21 examples of basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) in frozen tissue in a way equivalent to immunostains being applied to permanent sections. CONCLUSION: The 19-minute CK immunohistochemistry protocol in frozen tissue appears to be as effective at labeling tumor cells of SCC and BCC as methods requiring permanent sections. It is hopeful that this technique may prevent recurrences after MMS and limit the number of Mohs layers required to obtain free margins when inflammation is abundant. It also is effective in uncovering subtle perineural invasion. |
File Format | HTM / HTML |
ISSN | 10760512 |
Issue Number | 7 |
Volume Number | 35 |
e-ISSN | 15244726 |
Journal | Dermatologic Surgery |
Language | English |
Publisher | Lippincott Williams & Wilkins |
Publisher Date | 2009-07-01 |
Publisher Place | United States |
Access Restriction | Subscribed |
Subject Keyword | Discipline Dermatology Carcinoma, Basal Cell Pathology Carcinoma, Squamous Cell Frozen Sections Methods Immunohistochemistry Keratins Mohs Surgery Skin Neoplasms Aged, 80 And Over Antibodies, Monoclonal Antigens, Neoplasm Immunology Surgery Humans Male Staining And Labeling Time Factors Journal Article |
Content Type | Text |
Resource Type | Article |
Subject Domain (in MeSH) | Eukaryota Neoplasms Macromolecular Substances Amino Acids, Peptides, and Proteins Biological Factors Diagnosis Surgical Procedures, Operative Physical Phenomena Persons |
Subject | Dermatology Surgery |
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