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  1. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
  2. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
  3. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85, Issue 3, September 2015
  4. Bioprospecting of Thermotolerant Bacteria from Hot Water Springs of Himachal Pradesh for the Production of Taq DNA Polymerase
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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85, Issue 4, December 2015
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85, Issue 3, September 2015
Effects of Feeder Layers, Culture Media, Conditional Media, Growth Factors, and Passages Number on Stem Cell Optimization
Toxic Effect of Fluoride on Biochemical Parameters and Collagen Metabolism in Osseous and Non-Osseous Tissues of Rats
Optimising Elicitors and Precursors to Enhance Alizarin and Purpurin Production in Adventitious Roots of Morinda citrifolia L.
Production of Extracellular Isoamylase and Endoglucanase by Entrapped Spores of Rhizopus oryzae PR7
Bioprospecting of Thermotolerant Bacteria from Hot Water Springs of Himachal Pradesh for the Production of Taq DNA Polymerase
Purification and Characterization of Extracellular Phytase from Bacillus licheniformis Isolated from Fish Gut
Dietary Administered Bacillus sp. PP9 Enhances Growth, Nutrition and Immunity in Cirrhinus mrigala (Hamilton)
Influence of Processing Variables and Storage on the Fruit Drink Developed from Taktir; Wild Fruit of Himalaya
Dietary Fat Intake and Risk of Cardiovascular Diseases in Young Adult Males
Physico-Chemical Characteristics of Maize and Sorghum as Affected by Popping
Association of Clinical and Genetic Features in Familial Mediterranean Fever Among Children from Central Anatolia, Turkey
Expression of Erythroblastic Leukemia Viral Oncogene Homolog 2 (ERBB2) from Canine Mammary Tumor
Protein Kinase C Plays an Important Role in Exaggerated Vasoconstriction Associated with Insulin Deficiency but not Resistance
Rapid Detection of Avian Infectious Bronchitis Virus by Reverse Transcriptase-Loop Mediated Isothermal Amplification
Cellular and Nuclear Toxicity of HgCl$_{2}$ to In Vitro Grown Lymphocytes from Human Umbilical Cord Blood
Anti-inflammatory Activity of Emu Oil in Indomethacin Induced Inflammatory Bowel Disease in Rats
Comparative Analysis of Cultural and PCR Based Assays for Detection of Campylobacter spp. in Human Stool Samples
Pharmacokinetics of Enrofloxacin in Emu (Dromaius novaehollandiae) Birds After Intravenous and Oral Bolus Administration
Cloning, Expression and Characterisation of Recombinant Outer Membrane Protein 16 from Brucella spp.
Doppler Ultrasonography of Common Carotid Artery and External Jugular Vein in Healthy and Reticulo-Diaphragmatic Hernia Affected Buffaloes
Industrial Fluorosis and Its Effects on Serum Biochemistry and Haemogram in Cattle of Kerala, India
Recombinant LipL32 Based Enzyme Linked Immunosorbent Assay for Seroprevalence Studies of Leptospirosis in Livestock Farmers
Histopathological Studies Targeting Comparative Efficacy of Herbal and Standard Allopathic Immunomodulators Against Visceral Larva Migrans
Seroprevalence of Babesiosis and Anaplasmosis in Apparently Healthy Large Ruminants of Punjab, India
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85, Issue 2, June 2015
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85, Issue 1, March 2015
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 82

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“Isolation & characterization of Thermus aquaticus and production of Taq DNA polymerse enzymeâ€

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BIOPROSPECTING OF THERMOTOLERANT BACTERIA FROM HOT WATER SPRINGS OF HIMACHAL PRADESH FOR PRODUCTION OF THERMOSTABLE LIPASE ENZYME

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BIOPROSPECTING OF THERMOTOLERANT BACTERIA FROM HOT WATER SPRINGS OF HIMACHAL PRADESH FOR PRODUCTION OF THERMOSTABLE PULLULANASE ENZYME

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Isolation & characterization of Thermus aquaticus and production of Taq DNA polymerse enzyme

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Bioprospecting and molecular characterization of laccase producing bacteria from paper mills of Himachal Pradesh

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PRODUCTION, PURIFICATION AND CHARACTERIZATION OF Taq DNA POLYMERASE FROM Thermus aquaticus

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BIOPROSPECTING OF THERMOTOLERANT BACTERIA FROM HOT WATER SPRINGS OF H.P. FOR PRODUCTION OF THERMOSTABLE PROTEASE ENZYME

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Accuracy of replication in the polymerase chain reaction. Comparison between Thermotoga maritima DNA polymerase and Thermus aquaticus DNA polymerase

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Bioprospecting of thermophilic bacteria from hot water springs of Himachal Pradesh for laccase enzyme production

Thesis

Bioprospecting of Thermotolerant Bacteria from Hot Water Springs of Himachal Pradesh for the Production of Taq DNA Polymerase

Content Provider SpringerLink
Author Shirkot, Poonam Dhiman, Karuna Gupta, Monika Verma, Ambika
Copyright Year 2014
Abstract Thermophilic microorganisms are adapted to live at high temperatures and produce many thermostable enzymes such as Taq polymerase, amylase, lipase, protease, etc., which find a number of commercial applications because of their thermostability. Thermal springs of Manikaran, Vashisht, Khirganga, Tattapani and Jeory of districts Kullu, Mandi and Shimla respectively, were selected for the present study and forty two thermophilic bacterial isolates were isolated using Castenholz trypticase yeast extract medium. All these thermophilic bacterial isolates were screened using morphological and biochemical characters. For molecular characterization of the selected isolates, Polymerase chain reaction was carried out for the amplification of the DNA using genus specific primers for 16S ribosomal RNA gene. Nucleotide sequences obtained after sequencing were blasted and only two isolates were found to belong to genus Thermus and the strain MS3 was found to possess maximum homology of 99 % with Thermus aquaticus. The growth parameters optimized were found to be pH of 7.5, incubation temperature 65 °C and incubation time 96 h. Taq polymerase produced using selected strain MS3, was purified by ammonium sulphate precipitation, gel permeation chromatography and ion exchange chromatography. The Taq polymerase assay with 1.5 µl purified enzyme fraction obtained after ion exchange chromatography, yielded comparable results with 0.5 µl (3.0 U/µl) of commercial Taq DNA polymerase, indicating that the protein fraction has 1.0 U/µl of DNA polymerase activity. Molecular mass of the purified Taq DNA polymerase was found to be 94,000 daltons. Protein sequencing analysis of the purified enzyme product showed 99 % homology with A-Chain structure of Taq DNA polymerase with accession number 1TAQ A.
Starting Page 739
Ending Page 749
Page Count 11
File Format PDF
ISSN 03698211
Journal Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number 85
Issue Number 3
e-ISSN 22501746
Language English
Publisher Springer India
Publisher Date 2014-09-06
Publisher Place New Delhi
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Life Sciences Plant Biochemistry Nucleic Acid Chemistry Thermus aquaticus 16S ribosomal RNA Behavioral Sciences Thermal springs Thermophiles Taq DNA polymerase
Content Type Text
Resource Type Article
Subject Environmental Science Agricultural and Biological Sciences
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