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  1. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
  2. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
  3. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 2, June 2017
  4. Production of Polyhydroxyalkanoates (PHAs) by Bacillus Strain Isolated from Waste Water and Its Biochemical Characterization
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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 4, December 2017
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 3, September 2017
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 2, June 2017
Chromosome Complements of Isodon (Family: Lamiaceae–Subfamily: Nepetoideae): Trends in Karyotype Evolution
Molecular Detection of Reticuloendotheliosis Virus (REV) Integration in Avian Poxvirus in North Eastern India
Groundnut Meal and Carrot Fortified Pasta: Optimization of Ingredients Level Using RSM
Efficacy of Silicon Formulations on Sugarcane Stalk Borers, Quality Characteristics and Parasitism Rate on Five Commercial Varieties
Identifying microRNAs relating to morphine response in BE(2)-C cell line by microRNA profiling
Nutritional Requirements to Improve Delta-Endotoxins Production of Bacillus thuringiensis var. kurstaki Using Mixed Designs Modelling
Conservation and Cultivation of Ferula jaeschkeana Vatke: A Species with Deep Complex Morphophysiological Dormancy
Size-Class and Sex Dependent Variations of Burrow Architecture of Fiddler Crab, Uca rosea (Tweedie, 1937) in Sundarbans
Bioconsortia Augments Antioxidant and Yield in Matricaria recutita L. Against Meloidogyne incognita (Kofoid and White) Chitwood Infestation
Variation in Mycosis of Entomopathogenic Fungi on Mealybug, Paracoccus marginatus (Homoptera: Pseudococcidae)
Root Biomass Partitioning, Differential Antioxidant System and Thiourea Spray are Responsible for Heat Tolerance in Spring Maize
Molecular Detection and Assessment of Hemato-Biochemistry, Oxidant/Antioxidant Status in Natural Canine Monocytic Ehrlichiosis Cases from Northern India
Comparative Studies on Population Growth of Two Brachionus calyciflorus Sibling Species
Assessment of Water Status in Wheat (Triticum aestivum L.) Using Ground Based Hyperspectral Reflectance
Growth, Physiology and Biochemical Responses of Two Different Brassica Species to Elevated CO$_{2}$
Species Composition of Ants in Cashew Plantations and their Interrelationships with Cashew
Purification and bio-chemical characterization of a solvent-tolerant and highly thermostable lipase of Bacillus licheniformis strain SCD11501
Effects of Arbuscular Mycorrhizal Inoculations and Cotyledon Removal on Early Seedling Growth of Jatropha Curcas L.
Quercetin protects mice kidney collagen against the damage induced by cadmium fluoride
Nutritional Evaluation of Seaweed on Nutrient Digestibility, Nitrogen Balance, Milk Production and Composition in Sahiwal Cows
Studies on Lichens of Mizoram, Northeast India
Production of Polyhydroxyalkanoates (PHAs) by Bacillus Strain Isolated from Waste Water and Its Biochemical Characterization
Effect of Distillery Effluent, Biomethanated Spent Wash, on Microbial Activity Parameters in Soil Under Rice–Wheat Cropping System
Site Specific Nutrient Management of an Intensively Cultivated Farm Using Geostatistical Approach
Shade Trees Improve Soil Biological and Microbial Diversity in Coffee Based System in Western Ghats of India
Antibacterial Activity of Streptomyces sp. VITMK1 Isolated from Mangrove Soil of Pichavaram, Tamil Nadu, India
Homogenous PCR of Heterogeneous DNA from Phenolic Rich Barks of Terminalia Species for DNA Based Adulteration Detection
Effect of Waterlogging on Element Concentrations, Growth and Yield of Wheat Varieties Under Farmer’s Sodic Field Conditions
In Vitro Antimicrobial and Antioxidant Activity of Camelina Seed Extracts as Potential Source of Bioactive Compounds
Antifungal Molecules Produced by a New Salt-Tolerant and Alkaliphilic Streptomyces sp. BS30 Isolated from an Arid Soil
Biofilm Inhibitory Effect of Spirulina platensis Extracts on Bacteria of Clinical Significance
Comparative Karyological Findings in Native and Crossbred Pigs
Screening for Resistance Against Pulse Beetle, Callosobruchus analis (F.) in Greengram (Vigna radiata (L.) Wilczek) Accessions
Chemical Constituents, Antioxidative and Antibacterial Properties of Medicinal Mushrooms Collected from Similipal Biosphere Reserve, Odisha, India
Characteristics of Metabolic Changes and Antioxidative Response in a Potential Zinc Tolerant Fungal Strain, Aspergillus terreus
SCMR: A More Pertinent Trait than SLA in Peanut Genotypes Under Transient Water Deficit Stress During Summer
In Vitro Selection of Cell Lines in Punica granatum L. (Daru) Against Bacterial Blight
Antagonistic Behavior of Different Bioagents Against Dominant Seed Borne Fungi of Mungbean Seeds Under In Vitro Condition
Enhanced Production of Nitrilase from Streptomyces sp. MTCC 7546 by Response Surface Method
Toxicological Assessment and Ameliorative Effects of Parinari curatellifolia Alkaloids on Triton-Induced Hyperlipidemia and Atherogenicity in Rats
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87, Issue 1, March 2017
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 82

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Production of Polyhydroxyalkanoates (PHAs) by Bacillus Strain Isolated from Waste Water and Its Biochemical Characterization

Content Provider SpringerLink
Author Mohapatra, S. Sarkar, B. Kumar, C. Samantaray, D. P. Mohanta, P. R. Daware, A.
Copyright Year 2015
Abstract Municipal sewage triggers a stress prone environment to accumulate polyhydroxyalkanoates (PHAs) in the cytosol of bacteria. In view of that, different Bacillus species were isolated from municipal sewage and screened for evaluating their efficacy of PHA production. Growth parameters such as temperature, pH, glucose concentration and carbon nitrogen combinations were optimized with respect to higher biomass production as it is analogous to PHA accumulation. Under optimized conditions, the Bacillus species produced 3.09 g/L of PHAs which was estimated as a higher yield in comparison to other similar strains. Fourier transform infrared spectroscopic analysis of the extracted polyhydroxybutyrate confirmed the distinct peak corresponding to C=O group, whereas proton nuclear magnetic resonance ($^{1}$H NMR) and differential scanning colorimetric analysis exhibited detailed insight of its chemical structure and properties by reflecting monomeric unit. The high yielding bacterial isolate was identified by 16S rDNA sequencing and the sequence was confirmed as Bacillus subtilis with an accession no. KP172548 after submission to NCBI data base. The potential bacterium may be further exploited for cost effective and mass scale production of biopolymer.
Starting Page 459
Ending Page 466
Page Count 8
File Format PDF
ISSN 03698211
Journal Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number 87
Issue Number 2
e-ISSN 22501746
Language English
Publisher Springer India
Publisher Date 2015-07-31
Publisher Place New Delhi
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Life Sciences NMR Plant Biochemistry Bio-plastic Nucleic Acid Chemistry Behavioral Sciences Bacillus Polyhydroxyalkanoates Waste water
Content Type Text
Resource Type Article
Subject Environmental Science Agricultural and Biological Sciences
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