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  1. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
  2. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
  3. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85, Issue 4, December 2015
  4. Generation of HSC-Like Cells from Human Embryonic Stem Cells by Inhibition of TGF-βR2 Signaling
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Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 87
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 86
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85, Issue 4, December 2015
Impact of Heavy Metal Toxicity on Hematology and Glycogen Status of Fish: A Review
Neglected Tropical Diseases: Trematodiases—The Indian Scenario
Possibilities of Improving Performance of Direct Seeded Rice Using Plant Growth Regulators: A Review
Molecular and Probiotic Functional Characterization of Lactobacillus spp. Associated with Traditionally Fermented Fish, Tungtap of Meghalaya in Northeast India
Emergence of Aggressive Population in the Bipolaris sorokiniana of Barley (Hordeum vulgare L.) Through Anastomosis
Abundance and Spatio-Temporal Distributional Pattern of Dotilla Myctiroides (Milne-Edwards) on Exposed Sandy Beach of Aksa, Mumbai
Scanning Electron Microscopy and Molecular Characterization of Aedes albopictus (Skuse)
Functional Screening and Molecular Characterization of Halophilic and Halotolerant Bacteria by 16S rRNA Gene Sequence Analysis
Functional Characterization of Bubaline Recombinant-Cathelicidin
Molecular Characterization of Commercial Varieties of Phyllanthus emblica Using RAPD and Nuclear rDNA SNPs
Evaluation of Probiotic Potential of New Bacterial Strain, Lactobacillus spicheri G2 Isolated from Gundruk
Effect of Preconceptional Exposure of Triazophos Formulation on Fertility and Reproductive Performance of Female Wistar Rats, Rattus norvegicus
Characterisation and Validation of House Keeping Gene for Expression Analysis in Catla catla (Hamilton)
Transferability of SSR Markers of Brassica sp. to Some Popular Varieties of Brassica Juncea
Development of Novel Microsatellite Markers in Jatropha curcas and Evaluation of Their Cross-Species Transferability
Generation of HSC-Like Cells from Human Embryonic Stem Cells by Inhibition of TGF-βR2 Signaling
Analysis of Genetic Diversity in 21 Genotypes of Indian Banana Using RAPDs and IRAPs Markers
Chloroplast and Mitochondrial DNA Assay in Solving Issues Related to the Taxonomy of Beveragial Tea Clones
Effects of Phenanthrene and Pyrene on Cytogenetic Stability of Human Dermal Fibroblasts Using Alkaline Comet Assay Technique
In-Silico Designing of Molecular Beacon Probes for Sensitive and Rapid Detection of Water Borne Pathogenic Bacteria
Bactericidal Activity of Nanopolymers Against Shrimp Pathogenic Bacterium Vibrio harveyi
Biochemical Characterization of a RIL Population of Rice (Oryza sativa L.) Under Direct-Seeded Aerobic and Transplanted Conditions
Bioprospecting and molecular characterization of laccase producing bacteria from paper mills of Himachal Pradesh
Guanine and Cytosine at the Second Codon Position Influence Gene Expression in Cereals
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85, Issue 3, September 2015
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85, Issue 2, June 2015
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 85, Issue 1, March 2015
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 84
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 83
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences : Volume 82

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Generation of HSC-Like Cells from Human Embryonic Stem Cells by Inhibition of TGF-βR2 Signaling

Content Provider SpringerLink
Author Kazemi Sheykhshabani, Sedigheh Hashemzadeh Chaleshtori, Morteza Khosravi Farsani, Somayeh Ghasemi Dehkordi, Payam Ganji Arjenaki, Mahboubeh Abdian, Narges Allahbakhshian Farsani, Mehdi
Copyright Year 2015
Abstract The efficient induction of embryonic stem cells into hematopoietic stem cells (HSCs) requires understanding the pro or inhibitory regulatory networks, which are involved in these pathways. Transforming growth factor β family is a super family of growth factors such as TGF-β1, TGF-β2, TGF-β3, and some other cytokines. Previous works indicate that some of TGF-β subfamilies have negative regulatory effect during hematopoietic differentiation and HSC proliferation. The inhibitory effects of TGF-β1 in early stage of differentiation of endothelial and hematopoietic cells have been shown. Therefore, in the present study, in order to evaluate the promotion of hematopoietic differentiation, the activities of TGF-β regulatory factor were reduced through knockdown of TGF-βR2 expression. The ESCs were transduced by Lentivirus carrying SiTGF-βR2 and its scramble separately. Green fluorescent protein (GFP) was used as a marker to monitor gene expression. Six days post transduction GFP$^{+}$ colonies were used for EB generation and the next day, the same size EBs were transferred in differentiation media involved in defining cytokines. The expression level of hematopoietic-specific genes was evaluated in 0, 7, and 15-days of differentiation. The real-time PCR analysis in 15 day EBs showed 2–3-folds increase in SiTGF-βR2 as compared with the scramble. Therefore, this finding may be confirmed if TGF receptor silencing can reduce the inhibitory effect of TGF-β during early hematopoietic differentiation.
Starting Page 1017
Ending Page 1026
Page Count 10
File Format PDF
ISSN 03698211
Journal Proceedings of the National Academy of Sciences, India Section B: Biological Sciences
Volume Number 85
Issue Number 4
e-ISSN 22501746
Language English
Publisher Springer India
Publisher Date 2015-04-08
Publisher Place New Delhi
Access Restriction One Nation One Subscription (ONOS)
Subject Keyword Life Sciences Plant Biochemistry Nucleic Acid Chemistry TGF-βR2 signaling Behavioral Sciences HSCs ESCs
Content Type Text
Resource Type Article
Subject Environmental Science Agricultural and Biological Sciences
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