NDLI: Production of β-carotene by recombinant Escherichia coli with engineered whole mevalonate pathway in batch and fed-batch cultures

Content Provider	SpringerLink
Author	Kim, Jung Hun Kim, Seon Won Nguyen, Do Quynh Anh Li, He Kim, Sung Bae Seo, Yang Gon Yang, Jae Kyung Chung, In Young Kim, Dae Hwan Kim, Chang Joon
Copyright Year	2009
Abstract	Recombinant Escherichia coli engineered to contain the whole mevalonate pathway and foreign genes for β-carotene biosynthesis, was utilized for production of β-carotene in bioreactor cultures. Optimum culture conditions were established in batch and pH-stat fed-batch cultures to determine the optimal feeding strategy thereby improving production yield. The specific growth rate and volumetric productivity in batch cultures at 37°C were 1.7-fold and 2-fold higher, respectively, than those at 28°C. Glycerol was superior to glucose as a carbon source. Maximum β-carotene production (titer of 663 mg/L and overall volumetric productivity of 24.6 mg/L × h) resulted from the simultaneous addition of 500 g/L glycerol and 50 g/L yeast extract in pH-stat fed-batch culture.
Starting Page	559
Ending Page	564
Page Count	6
File Format	PDF
ISSN	12268372
Journal	Biotechnology and Bioprocess Engineering
Volume Number	14
Issue Number	5
e-ISSN	19763816
Language	English
Publisher	The Korean Society for Biotechnology and Bioengineering
Publisher Date	2009-11-05
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	recombinant Escherichia coli engineered whole mevalonate pathway β-carotene bioreactor culture fed-batch culture Industrial and Production Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Biotechnology Biomedical Engineering Bioengineering Applied Microbiology and Biotechnology

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