NDLI: Development of cell culture system from the giant freshwater prawn Macrobrachium rosenbergii (de Man)

Content Provider	SpringerLink
Author	Goswami, Mukunda Lakra, Wazir S. Rajaswaminathan, T. Rathore, Gourav
Copyright Year	2009
Abstract	A new cell culture system (MRH) was developed for the first time from 2 months old freshwater prawn, Macrobrachium rosenbergii. Primary cultures were developed from heart tissues by explant culture technique. Cell outgrowth was obtained from the heart explant after 14 days of explant culture. The culture medium used was Leibovitz-15 supplemented with 20% Fetal Bovine Serum along with 1% prawn hemolymph serum, 0.1% glucose, 0.5% NaCl and antibiotics (Penicillin 10,000 Units ml$^{−1}$, Streptomycin 10,000 μg ml$^{−1}$, Amphotericin B 500 mg ml$^{−1}$) with a final osmomolality of 470–550 mmol kg$^{−1}$. The pH of the growth medium found suitable for the growth of the cells was 7.20. The viability of cells was found to be 60% when revived after a month of storage in liquid nitrogen.
Starting Page	2043
Ending Page	2048
Page Count	6
File Format	PDF
ISSN	03014851
Journal	Molecular Biology Reports
Volume Number	37
Issue Number	4
e-ISSN	15734978
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-07-31
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Macrobrachium rosenbergii Cell culture Giant freshwater prawn MRH Animal Biochemistry Animal Anatomy / Morphology / Histology
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Genetics Medicine

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Development of cell culture system from the giant freshwater prawn Macrobrachium rosenbergii (de Man)