NDLI: Unmodified calcium concentrations in tumour necrosis factor receptor subtype-mediated apoptotic cell death

Content Provider	SpringerLink
Author	McFarlane, Shona M. Anderson, Helen M. Tucker, Steven J. Jupp, Orla J. McEwan, David J.
Copyright Year	2000
Abstract	Tumour necrosis factor-α (TNF) receptors mediate a variety of effects dependent on cell type. A role for Ca$^{2+}$ in TNF-induced death remains uncertain. Here we investigated restricting intracellular/extracellular Ca$^{2+}$ in HeLa epithelial carcinoma cells expressing low and high levels of p75TNFR receptor subtype and KYM-1 rhabdomyosarcoma cells, models of rapid TNF-induced apoptosis. Ca$^{2+}$-chelators EGTA and BAPTA-AM as well as microsomal Ca$^{2+}$-ATPase inhibitor thapsigargin, did not alter TNF-induced death. TNF was also unable to alter resting [Ca$^{2+}$]$_{i}$ levels which remained < 200 nM even during times when these cells were undergoing apoptotic cell death. These findings indicate no role for modulated Ca$^{2+}$ concentrations in TNF-induced apoptotic cell death.
Starting Page	19
Ending Page	26
Page Count	8
File Format	PDF
ISSN	03008177
Journal	Molecular and Cellular Biochemistry
Volume Number	211
Issue Number	1-2
e-ISSN	15734919
Language	English
Publisher	Kluwer Academic Publishers
Publisher Date	2000-01-01
Publisher Place	Boston
Access Restriction	Subscribed
Subject Keyword	Cardiology Medical Biochemistry Oncology Biochemistry
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Clinical Biochemistry Cell Biology Medicine

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Unmodified calcium concentrations in tumour necrosis factor receptor subtype-mediated apoptotic cell death