NDLI: Increasing Hematopoietic Stem Cell Yield to Develop Mice with Human Immune Systems

Content Provider	PubMed Central
Author	Biancotti, Juan-carlos Town, Terrence
Copyright Year	2013
Abstract	Hematopoietic stem cells (HSCs) are unique in their capacity to give rise to all mature cells of the immune system. For years, HSC transplantation has been used for treatment of genetic and neoplastic diseases of the hematopoietic and immune systems. The sourcing of HSCs from human umbilical cord blood has salient advantages over isolation from mobilized peripheral blood. However, poor sample yield has prompted development of methodologies to expand HSCs ex vivo. Cytokines, trophic factors, and small molecules have been variously used to promote survival and proliferation of HSCs in culture, whilst strategies to lower the concentration of inhibitors in the culture media have recently been applied to promote HSC expansion. In this paper, we outline strategies to expand HSCs in vitro, and to improve engraftment and reconstitution of human immune systems in immunocompromised mice. To the extent that these “humanized” mice are representative of the endogenous human immune system, they will be invaluable tools for both basic science and translational medicine.
Related Links	http://dx.doi.org/10.1155/2013/740892
Starting Page	740892
File Format	PDF
ISSN	23146133
e-ISSN	23146141
Journal	BioMed Research International
Volume Number	2013
Language	English
Publisher	Hindawi Publishing Corporation
Publisher Date	2013-01-01
Access Restriction	Open
Rights Holder	Hindawi Publishing Corporation
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Medicine Immunology and Microbiology Biochemistry, Genetics and Molecular Biology

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Increasing Hematopoietic Stem Cell Yield to Develop Mice with Human Immune Systems

Albumin-expressing hepatocyte-like cells develop in the livers of immune-deficient mice that received transplants of highly purified human hematopoietic stem cells

Definitive hematopoietic stem cells first develop within the major arterial regions of the mouse embryo

All hematopoietic cells develop from hematopoietic stem cells through Flk2/Flt3-positive progenitor cells

Fatal autoimmunity in mice reconstituted with human hematopoietic stem cells encoding defective FOXP3

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The neural crest is a source of mesenchymal stem cells with specialized hematopoietic stem cell niche function

‘From blood to blood’: de-differentiation of hematopoietic progenitors to stem cells

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Increasing Hematopoietic Stem Cell Yield to Develop Mice with Human Immune Systems

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Increasing Hematopoietic Stem Cell Yield to Develop Mice with Human Immune Systems

Albumin-expressing hepatocyte-like cells develop in the livers of immune-deficient mice that received transplants of highly purified human hematopoietic stem cells

Definitive hematopoietic stem cells first develop within the major arterial regions of the mouse embryo

All hematopoietic cells develop from hematopoietic stem cells through Flk2/Flt3-positive progenitor cells

Fatal autoimmunity in mice reconstituted with human hematopoietic stem cells encoding defective FOXP3

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