NDLI: Transcription-dependent R-loop formation at mammalian class switch sequences

Content Provider	PubMed Central
Author	Tracy, Robert B. Lieber, Michael R.
Copyright Year	2000
Abstract	Immunoglobulin class switching is mediated by recombination between switch sequences located immediately upstream of the immunoglobulin constant heavy chain genes. Targeting of recombination to particular switch sequences is associated temporally with transcription through these regions. We recently have provided evidence for inducible and stable RNA–DNA hybrid formation at switch sequences in the mouse genome that are mechanistically important for class switching in vivo. Here, we define in vitro the precise configuration of the DNA and RNA strands within this hybrid structure at the Sμ, Sγ3 and Sγ2b mouse switch sequences. We find that the G–rich (non-template) DNA strand of each switch sequence is hypersensitive to probes throughout much of its length, while the C–rich (template) DNA strand is essentially resistant. These results demonstrate formation of an R–loop, whereby the G–rich RNA strand forms a stable heteroduplex with its C–rich DNA strand counterpart, and the G–rich DNA strand exists primarily in a single-stranded state. We propose that the organized structure of the R–loop is essential for targeting the class switch recombination machinery to these sequences.
Related Links	http://dx.doi.org/10.1093/emboj/19.5.1055
Ending Page	1067
Page Count	13
Starting Page	1055
File Format	PDF
ISSN	02614189
e-ISSN	14602075
Journal	The EMBO Journal
Issue Number	5
Volume Number	19
Language	English
Publisher	Oxford University Press
Publisher Date	2000-03-01
Access Restriction	Open
Rights Holder	Oxford University Press
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Immunology and Microbiology(all) Neuroscience(all) Molecular Biology Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Neuroscience Medicine Immunology and Microbiology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Libarray of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Transcription-dependent R-loop formation at mammalian class switch sequences.

pH-dependent conformational switch activates the inhibitor of transcription elongation

Sense transcription through the S region is essential for immunoglobulin class switch recombination

Three classes of mammalian transcription activation domain stimulate transcription in Schizosaccharomyces pombe.

Id helix-loop-helix proteins inhibit nucleoprotein complex formation by the TCF ETS-domain transcription factors.

Mechanism of R-Loop Formation at Immunoglobulin Class Switch Sequences▿ †

Redox-switch modulation of human SSADH by dynamic catalytic loop

Inhibition of the mammalian transcription factor LSF induces S-phase-dependent apoptosis by downregulating thymidylate synthase expression

Transcription activation at Class II CRP-dependent promoters: identification of determinants in the C-terminal domain of the RNA polymerase alpha subunit.

Transcription-dependent R-loop formation at mammalian class switch sequences

Similar Documents

Transcription-dependent R-loop formation at mammalian class switch sequences.

pH-dependent conformational switch activates the inhibitor of transcription elongation

Sense transcription through the S region is essential for immunoglobulin class switch recombination

Three classes of mammalian transcription activation domain stimulate transcription in Schizosaccharomyces pombe.

Id helix-loop-helix proteins inhibit nucleoprotein complex formation by the TCF ETS-domain transcription factors.

Mechanism of R-Loop Formation at Immunoglobulin Class Switch Sequences▿ †

Redox-switch modulation of human SSADH by dynamic catalytic loop

Inhibition of the mammalian transcription factor LSF induces S-phase-dependent apoptosis by downregulating thymidylate synthase expression

Transcription activation at Class II CRP-dependent promoters: identification of determinants in the C-terminal domain of the RNA polymerase alpha subunit.

Transcription-dependent R-loop formation at mammalian class switch sequences