Please wait, while we are loading the content...
Please wait, while we are loading the content...
Content Provider | Journal of Biological Chemistry (JBC) |
---|---|
Author | Zhu, Ying Saunders, Michael A. Yeh, Howard Deng, Wu-guo Wu, Kenneth K. |
Abstract | To elucidate the mechanism by which isoforms of CCAAT/enhancer-binding proteins regulate cyclooxygenase-2 expression, we determined by a novel technique binding of six isoforms of this transactivator to two sequence-specific CCAAT/enhancer-binding protein (−132/−125) and cyclic AMP (−59/−53) regulatory elements in human foreskin fibroblasts treated with phorbol 12-myristate 13-acetate for 4 h. The δ isoform bound to these two elements at basal state, which was displaced by full-length as well as two truncated β isoforms, a 41-kDa liver-enriched activating protein and a 16-kDa liver-enriched inhibitory protein, after phorbol ester stimulation. Kinetic analysis shows time-dependent changes in β and δ binding that were concordant with time-dependent increase in cyclooxygenase-2 induction. Overexpression of the 16-kDa β isoform blocked the promoter activity and protein level induced by phorbol ester. Paradoxically, it increased binding of β isoforms to the sequence-specific promoter DNA but suppressed cyclooxygenase-2 promoter activation by p300 cotransfection. These findings provide new insight into the regulation of cyclooxygenase-2 promoter by an interplay between two opposite β isoforms and p300 co-activator. |
Related Links | http://www.jbc.org/content/277/9/6923.abstract |
Ending Page | 6928 |
Starting Page | 6923 |
Page Count | 6 |
File Format | HTM / HTML PDF |
ISSN | 00219258 |
Journal | Journal of Biological Chemistry (JBC) |
Issue Number | 9 |
Volume Number | 277 |
DOI | 10.1074/jbc.M108075200 |
e-ISSN | 1083351X |
Language | English |
Publisher | American Society for Biochemistry and Molecular Biology |
Publisher Date | 2002-03-01 |
Access Restriction | Open |
Subject Keyword | Cyclooxygenase-2 (COX-2) CCAAT/enhancer-binding protein (C/EBP) Phorbol 12-myristate 13-acetate (PMA) Liver-enriched activating protein (LAP) Liver-enriched inhibitory protein (LIP) Human foreskin fibroblasts (HFF) Cyclic AMP responsive element (CRE) Full-length C/EBPβ (C/EBPβ-FL) GENES: STRUCTURE AND REGULATION |
Content Type | Text |
Resource Type | Article |
Subject | Molecular Biology Cell Biology Biochemistry |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
Sl. | Authority | Responsibilities | Communication Details |
---|---|---|---|
1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
7 | National Digital Libarray of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
Loading...
|