Please wait, while we are loading the content...
Please wait, while we are loading the content...
Content Provider | Journal of Biological Chemistry (JBC) |
---|---|
Author | Celle, Peter T. La Polakowska, Renata R. |
Description | Class 1 homeobox transcription factors related to theDrosophila Antennapedia complex and Bithorax complex genes (HOX) |
Abstract | Keratinocyte proliferation and differentiation result from expression of specific groups of genes regulated by unique combinations of transcription factors. To better understand these regulatory processes, we studied HOXA7expression and its regulation of differentiation-specific keratinocyte genes. We isolated the homeobox transcription factor HOXA7 from keratinocytes through binding to a differentiation-dependent viral enhancer and analyzed its effect on endogenous differentiation-dependent genes, primarily transglutaminase 1. HOXA7 overexpression repressed transglutaminase 1-reporter activity. HOXA7 message markedly decreased, and transglutaminase RNA increased, upon phorbol ester-induced differentiation, in a protein kinase C-dependent manner. Overexpression of HOXA7 attenuated thetransglutaminase 1 induction by phorbol ester, demonstrating that HOXA7 expression is inversely related to keratinocyte differentiation, and to transglutaminase 1expression. Antisense HOXA7 expression activated transglutaminase 1, involucrin, and keratin 10 message and protein levels, demonstrating that endogenous HOXA7 down-regulates multiple differentiation-specific keratinocyte genes. In keeping with these observations, epidermal growth factor receptor activation stimulated HOXA7expression. HOX genes function in groups, and we found thatHOXA5 and HOXB7 were also down-regulated by phorbol ester. These results provide the first example of protein kinase C-mediated homeobox gene regulation in keratinocytes, and new evidence that HOXA7, potentially in conjunction with HOXA5 and HOXAB7, silences differentiation-specific genes during keratinocyte proliferation, that are then released from inhibition in response to differentiation signals. |
Related Links | http://www.jbc.org/content/276/35/32844.abstract |
Ending Page | 32853 |
Starting Page | 32844 |
Page Count | 10 |
File Format | HTM / HTML PDF |
ISSN | 00219258 |
Journal | Journal of Biological Chemistry (JBC) |
Issue Number | 35 |
Volume Number | 276 |
DOI | 10.1074/jbc.M104598200 |
e-ISSN | 1083351X |
Language | English |
Publisher | American Society for Biochemistry and Molecular Biology |
Publisher Date | 2001-08-31 |
Access Restriction | Open |
Subject Keyword | Epidermal growth factor (EGF) Transglutaminase 1 gene (TGM1) Transglutaminase type 1 (TGase1) A 1.7-kilobase pairTGM1 gene upstream regulatory DNA fragment (K3) Protein kinase C (PKC) Primary neonatal human keratinocytes (NHK) 12-O-tetradecanoylphorbol-13-acetate (TPA) Human papillomavirus 16 (HPV-16) Reverse transcription-polymerase chain reaction (RT-PCR) Activator protein 1 (AP-1) Activator protein 2 (AP-2) Transcription factor Sp1 (Sp1) Small proline rich-related peptide 2A (SPRR2A) Pit-Oct-Unc-related transcription factors containing a conserved homeodomain and POU domain (POU) Drosophiladistal-less-like homeobox transcription factor (Dlx3) CAMP-regulated enhancer binding-binding protein (CBP) Base pair(s) (bp) Polyacrylamide gel electrophoresis (PAGE) 4-morpholinepropanesulfonic acid (MOPS) Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) MOLECULAR BASIS OF CELL AND DEVELOPMENTAL BIOLOGY |
Content Type | Text |
Resource Type | Article |
Subject | Molecular Biology Cell Biology Biochemistry |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
Sl. | Authority | Responsibilities | Communication Details |
---|---|---|---|
1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
7 | National Digital Libarray of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
Loading...
|