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Content Provider | Journal of Biological Chemistry (JBC) |
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Author | Wielowieyski, Paul A. Wigle, Jeffrey T. Salih, Maysoon Hum, Peggy Tuana, Balwant S. |
Abstract | Novel splice variants of the α1 subunit of the Cav1.2 voltage-gated Ca2+ channel were identified that predicted two truncated forms of the α1 subunit comprising domains I and II generated by alternative splicing in the intracellular loop region linking domains II and III. In rabbit heart splice variant 1 (RH-1), exon 19 was deleted, which resulted in a reading frameshift of exon 20 with a premature termination codon and a novel 19-amino acid carboxyl-terminal tail. In the RH-2 variant, exons 17 and 18 were deleted, leading to a reading frameshift of exons 19 and 20 with a premature stop codon and a novel 62-amino acid carboxyl-terminal tail. RNase protection assays with RH-1 and RH-2 cRNA probes confirmed the expression in cardiac and neuronal tissue but not skeletal muscle. The deduced amino acid sequence from full-length cDNAs encoding the two variants predicted polypeptides of 99.0 and 99.2 kDa, which constituted domains I and II of the α1 subunit of the Cav1.2 channel. Antipeptide antibodies directed to sequences in the second intracellular loop between domains II and III identified the 240-kDa Cav1.2 subunit in sarcolemmal and heavy sarcoplasmic reticulum (HSR) membranes and a 99-kDa polypeptide in the HSR. An antipeptide antibody raised against unique sequences in the RH-2 variant also identified a 99-kDa polypeptide in the HSR. These data reveal the expression of additional Ca2+ channel structural units generated by alternative splicing of the Ca v 1.2 gene. |
Related Links | http://www.jbc.org/content/276/2/1398.abstract |
Ending Page | 1406 |
Starting Page | 1398 |
Page Count | 9 |
File Format | HTM / HTML PDF |
ISSN | 00219258 |
Journal | Journal of Biological Chemistry (JBC) |
Issue Number | 2 |
Volume Number | 276 |
DOI | 10.1074/jbc.M006868200 |
e-ISSN | 1083351X |
Language | English |
Publisher | American Society for Biochemistry and Molecular Biology |
Publisher Date | 2001-01-12 |
Access Restriction | Open |
Subject Keyword | Membrane-spanning regions 1–6 (S1–S6) Second intracellular loop between domains II and III (loop II–III) Reverse transcription-polymerase chain reaction (RT-PCR) Rabbit heart splice variant (RH) Nucleotide (nt) Base pair (bp) Sarcolemma (SL) Sarcoplasmic reticulum (SR) Heavy sarcoplasmic reticulum (HSR) Protein kinase C (PKC) Antipeptide antibody to loop II–III (CNC-1) MEMBRANE TRANSPORT STRUCTURE FUNCTION AND BIOGENESIS |
Content Type | Text |
Resource Type | Article |
Subject | Molecular Biology Cell Biology Biochemistry |
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