Please wait, while we are loading the content...
Please wait, while we are loading the content...
Content Provider | Royal Society of Chemistry (RSC) |
---|---|
Author | Groegel, Dominik B. M. Gorris, Hans H. Wolfbeis, Otto S. Wang, Xu-dong Stolwijk, Judith A. Meier, Robert J. Wegener, Joachim |
Copyright Year | 2011 |
Abstract | Intracellular oxygen is an important indicator for cell metabolism and respiration. We have designed self-referenced RGB PEBBLEs that enable a simple readout of the intracellular oxygen distribution with conventional wide-field microscopy and a standard RGB digital camera. The RGB PEBBLEs consist of a hydrophobic matrix covered with amino groups on the surface to confer water-dispersibility. Two luminophores are incorporated in a hydrophobic polystyrene matrix that is highly permeable to oxygen. In polystyrene, the dyes are largely protected from quenching or aggregation by cellular components. The dyes have been selected to match the green and the red channel of digital cameras. While the red emission of the oxygen probe is highly sensitive to oxygen with a quenching response of 74%, the green emission of the reference dye is stable under varying oxygen concentrations. Ratiometric images of intracellular oxygen have been acquired that are inherently resistant to fluctuations in absolute signal intensities. As RGB PEBBLEs respond within seconds to changing oxygen concentrations, they are amenable to monitoring fast cellular dynamics. |
Starting Page | 901 |
Ending Page | 906 |
Page Count | 6 |
File Format | HTM / HTML PDF |
ISSN | 20416520 |
Volume Number | 2 |
Issue Number | 5 |
Journal | Chemical Science |
DOI | 10.1039/c0sc00610f |
Language | English |
Publisher | Royal Society of Chemistry |
Access Restriction | Open |
Subject Keyword | Dye Oxygen Microscopy Cellular respiration Extracellular matrix Hydrophobe Metabolism Polystyrene RGB color model Digital camera |
Content Type | Text |
Resource Type | Article |
Subject | Chemistry |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
Sl. | Authority | Responsibilities | Communication Details |
---|---|---|---|
1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
7 | National Digital Libarray of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
Loading...
|