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Content Provider | SpringerLink |
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Author | Witten, Katrin G. Ruff, Julie Mohr, Anne Görtz, Dieter Recker, Tobias Rinis, Natalie Rech, Claudia Elling, Lothar Müller Newen, Gerhard Simon, Ulrich |
Copyright Year | 2013 |
Abstract | DNA-functionalized gold nanoparticles (AuNP–DNA) were hybridized with complementary di-N-acetyllactosamine-(di-LacNAc, [3Gal(β1-4)GlcNAc(β1-]2)-modified oligonucleotides to form glycol-functionalized particles, AuNP–DNA–di-LacNAc. While AuNP–DNA are known to be taken up by cells via scavenger receptors, glycol-functionalized particles have shown to be taken up via asialoglycoprotein receptors (ASGP-R). In this work, the interaction of these new particles with HepG2 cells was analyzed, which express scavenger receptors class B type I (SR-BI) and ASGP-R. To study the contribution of these receptors as potential mediators for cellular uptake, receptor-blocking experiments were performed with d-lactose, a ligand for ASGP-R, Fucoidan, a putative ligand for SR-BI, and a SR-BI blocking antibody. Labeling with Cy5-modified DNA ligands enabled us to monitor the particle uptake by confocal fluorescence microscopy and flow cytometry, in order to discriminate the two putative pathways by competitive binding studies. While SR-BI-antibody and d-lactose had no inhibiting effects on particle uptake Fucoidan led to a complete inhibition. Thus, a receptor-mediated uptake by the two receptors studied could not be proven and therefore other uptake mechanisms have to be considered. |
Starting Page | 1 |
Ending Page | 12 |
Page Count | 12 |
File Format | |
ISSN | 13880764 |
Journal | Journal of Nanoparticle Research |
Volume Number | 15 |
Issue Number | 10 |
e-ISSN | 1572896X |
Language | English |
Publisher | Springer Netherlands |
Publisher Date | 2013-09-22 |
Publisher Place | Dordrecht |
Access Restriction | Subscribed |
Subject Keyword | Gold nanoparticles DNA Carbohydrates Cellular uptake Hepatocytes Nanotechnology Inorganic Chemistry Characterization and Evaluation of Materials Physical Chemistry Optics, Optoelectronics, Plasmonics and Optical Devices |
Content Type | Text |
Resource Type | Article |
Subject | Bioengineering Atomic and Molecular Physics, and Optics Nanoscience and Nanotechnology Condensed Matter Physics Modeling and Simulation Chemistry |
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