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Content Provider | SpringerLink |
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Author | Jiang, Hong Bo Liu, Yong Hua Tang, Pei An Zhou, An Wei Wang, Jin Jun |
Copyright Year | 2009 |
Abstract | Four housekeeping genes named Lbβ-Actin1, Lbβ-Actin2, Lbα-Tubulin, and LbGapdh were cloned from Liposcelis bostrychophila using the combined techniques of reverse transcriptase-PCR (RT-PCR) with rapid amplification of cDNA ends (RACE). The GenBank accession numbers were FJ196622, FJ447483, FJ595242, and FJ595241, respectively. The full-length cDNA of Lbβ-Actin1 was a 1,772 bp sequence with an open reading frame (ORF) encoded 376 amino acids, while Lbβ-Actin2 was 1,350 bp in length containing an ORF encoded 376 amino acids. Furthermore, the 1,565 bp cDNA of Lbα-Tubulin had an ORF of 1,350 bp encoding 450 amino acids. And LbGapdh possessed an ORF of 333 amino acids. Sequences analysis and phylogenetic trees generated from the nucleotide sequences of their coding regions revealed a relationship that was closer to other insects than to mammals. The four genes together with 18S rRNA were quantified for transcription stability in L. bostrychophila, and the geNorm software ranked from the most to least were Lbβ-Actin1 > LbGapdh > Lbα-Tubulin > Lbβ-Actin2 > Lb18S rRNA for deltamethrin induction, while ranked Lbβ-Actin1 > Lbα-Tubulin > Lbβ-Actin2 > LbGapdh > Lb18S rRNA for the different developmental stages. |
Starting Page | 1019 |
Ending Page | 1029 |
Page Count | 11 |
File Format | |
ISSN | 03014851 |
Journal | Molecular Biology Reports |
Volume Number | 37 |
Issue Number | 2 |
e-ISSN | 15734978 |
Language | English |
Publisher | Springer Netherlands |
Publisher Date | 2009-09-13 |
Publisher Place | Dordrecht |
Access Restriction | Subscribed |
Subject Keyword | Psocoptera Insecticide induction Developmental expression Reference gene selection Animal Biochemistry Animal Anatomy / Morphology / Histology |
Content Type | Text |
Resource Type | Article |
Subject | Molecular Biology Genetics Medicine |
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