NDLI: Molecular cloning and characterization of a phytochelatin synthase gene, PvPCS1, from Pteris vittata L.

Content Provider	SpringerLink
Author	Dong, Ruibin Formentin, Elide Losseso, Carmen Carimi, Francesco Benedetti, Piero Terzi, Mario Schiavo, Fiorella Lo
Copyright Year	2005
Abstract	Pteris vittata L. is a staggeringly efficient arsenic hyperaccumulator that has been shown to be capable of accumulating up to 23,000 μg arsenic g−1, and thus represents a species that may fully exploit the adaptive potential of plants to toxic metals. However, the molecular mechanisms of adaptation to toxic metal tolerance and hyperaccumulation remain unknown, and P. vittata genes related to metal detoxification have not yet been identified. Here, we report the isolation of a full-length cDNA sequence encoding a phytochelatin synthase (PCS) from P. vittata. The cDNA, designated PvPCS1, predicts a protein of 512 amino acids with a molecular weight of 56.9 kDa. Homology analysis of the PvPCS1 nucleotide sequence revealed that it has low identity with most known plant PCS genes except AyPCS1, and the homology is largely confined to two highly conserved regions near the 5′-end, where the similarity is as high as 85–95%. The amino acid sequence of PvPCS1 contains two Cys-Cys motifs and 12 single Cys, only 4 of which (Cys-56, Cys-90/91, and Cys-109) in the N-terminal half of the protein are conserved in other known PCS polypeptides. When expressed in Saccharomyces cerevisae, PvPCS1 mediated increased Cd tolerance. Cloning of the PCS gene from an arsenic hyperaccumulator may provide information that will help further our understanding of the genetic basis underlying toxic metal tolerance and hyperaccumulation.
Starting Page	527
Ending Page	533
Page Count	7
File Format	PDF
ISSN	13675435
Journal	Journal of Industrial Microbiology and Biotechnology
Volume Number	32
Issue Number	11-12
e-ISSN	14765535
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-05-26
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Pteris vittata Phytochelatin Phytochelatin synthase Arsenic hyperaccumulation Heavy metal hypertolerance Microbial Genetics and Genomics Microbiology Bioinformatics Biochemistry Genetic Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Biotechnology Bioengineering Applied Microbiology and Biotechnology Medicine

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Molecular cloning and characterization of a phytochelatin synthase gene, PvPCS1, from Pteris vittata L.

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