NDLI: Supplementing l-leucine to a low-protein diet increases tissue protein synthesis in weanling pigs

Content Provider	SpringerLink
Author	Yin, Yulong Yao, Kang Liu, Zhaojin Gong, Min Ruan, Zheng Deng, Dun Tan, Bie Liu, Zhiqiang Wu, Guoyao
Copyright Year	2010
Abstract	Recent work with young pigs shows that reducing dietary protein intake can improve gut function after weaning but results in inadequate provision of essential amino acids for muscle growth. Because acute administration of l-leucine stimulates protein synthesis in piglet muscle, the present study tested the hypothesis that supplementing l-leucine to a low-protein diet may maintain the activation of translation initiation factors and adequate protein synthesis in multiple organs of post-weaning pigs. Eighteen 21-day pigs (Duroc × Landrace × Yorkshire) were fed low-protein diets (16.9% crude protein) supplemented with 0, 0.27 or 0.55% l-leucine (total leucine contents in the diets being 1.34, 1.61 or 1.88%, respectively). At 35 days of age, protein synthesis was determined using the [2H] phenylalanine flooding-dose technique. Additionally, total and phosphorylated levels of mammalian target of rapamycin (mTOR), ribosomal protein S6 kinase 1 (S6K1), and eIF4E-binding protein-1 (4E-BP1) were measured in longissimus muscle and liver. Compared with the control group, dietary supplementation with 0.55% l-leucine for 2 weeks increased (P < 0.05): (1) the phosphorylated levels of S6K1 and 4E-BP1; (2) protein synthesis in skeletal muscle, liver, the heart, kidney, pancreas, spleen, and stomach; and (3) daily weight gain by 61%. Dietary supplementation with 0.27% l-leucine enhanced (P < 0.05) protein synthesis in proximal small intestine, kidney and pancreas. These novel findings provide a molecular basis for designing effective nutritional means to increase the efficiency of nutrient utilization for protein accretion in neonates.
Starting Page	1477
Ending Page	1486
Page Count	10
File Format	PDF
ISSN	09394451
Journal	Amino Acids
Volume Number	39
Issue Number	5
e-ISSN	14382199
Language	English
Publisher	Springer Vienna
Publisher Date	2010-05-15
Publisher Place	Vienna
Access Restriction	Subscribed
Subject Keyword	Food Weanling pigs Protein synthesis Tissues Neurobiology Proteomics Life Sciences Biochemical Engineering Analytical Chemistry Biochemistry
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry Organic Chemistry Biochemistry

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