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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Haddad, Dania Boulianne, Bryant Gommerman, Jennifer L. Le, Michael X. Martin, Alberto Meng, Lingjin Ward, Lesley A. Li, Conglei |
Description | Country affiliation: Canada Author Affiliation: Boulianne B ( Department of Immunology, University of Toronto, Toronto, Ontario M5S 1A8, Canada.) |
Abstract | Upon activation with T-dependent Ag, B cells enter germinal centers (GC) and upregulate activation-induced deaminase (AID). AID(+) GC B cells then undergo class-switch recombination and somatic hypermutation. Follicular dendritic cells (FDC) are stromal cells that underpin GC and require constitutive signaling through the lymphotoxin (LT) ß receptor to be maintained in a fully mature, differentiated state. Although it was shown that FDC can be dispensable for the generation of affinity-matured Ab, in the absence of FDC it is unclear where AID expression occurs. In a mouse model that lacks mature FDC, as well as other LT-sensitive cells, we show that clusters of AID(+)PNA(+)GL7(+) Ag-specific GC B cells form within the B cell follicles of draining lymph nodes, suggesting that FDC are not strictly required for GC formation. However, later in the primary response, FDC-less GC dissipated prematurely, correlating with impaired affinity maturation. We examined whether GC dissipation was due to a lack of FDC or other LTß receptor-dependent accessory cells and found that, in response to nonreplicating protein Ag, FDC proved to be more critical for long-term GC maintenance. Our study provides a spatial-temporal analysis of Ag-specific B cell activation and AID expression in the context of a peripheral lymph node that lacks FDC-M1(+) CD35(+) FDC and other LT-sensitive cell types, and reveals that FDC are not strictly required for the induction of AID within an organized GC-like environment. |
ISSN | 00221767 |
e-ISSN | 15506606 |
Journal | The Journal of Immunology |
Issue Number | 9 |
Volume Number | 191 |
Language | English |
Publisher | The American Association of Immunologists |
Publisher Date | 2013-11-01 |
Publisher Place | United States |
Access Restriction | Open |
Subject Keyword | B-lymphocytes Immunology Cytidine Deaminase Metabolism Dendritic Cells, Follicular Germinal Center Cytology Animals Cell Differentiation Cells, Cultured Biosynthesis Lymph Nodes Lymphocyte Activation Lymphotoxin Beta Receptor Mice Mice, Inbred C57bl Mice, Knockout Receptors, Complement 3b Research Support, Non-u.s. Gov't Discipline Immunology |
Content Type | Text |
Resource Type | Article |
Subject | Immunology and Allergy Immunology |
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