Please wait, while we are loading the content...
Please wait, while we are loading the content...
Content Provider | World Health Organization (WHO)-Global Index Medicus |
---|---|
Author | Calvillo, I. Moreno, F. J. Sanz, M. L. Lebrón-Aguilar, R. Hernández-Hernández, O. |
Description | Country affiliation: Spain Author Affiliation: Hernández-Hernández O ( Instituto de Química Orgánica General (CSIC), C/Juan de la Cierva, 3 28006 Madrid, Spain.) |
Abstract | Three different stationary phases (sulfoalkylbetaine zwitterionic, polyhydroxyethyl aspartamide and ethylene bridge hybrid (BEH) with trifunctionally bonded amide), operating at hydrophilic interaction liquid chromatographic (HILIC) mode, have been assayed and compared for the analysis of complex mixtures of galactooligosaccharides (GOS). Chromatographic methods have been optimized to obtain the best separation between two consecutive galactose containing standards and maltodextrins, measured on the basis of resolution. Influence of several factors such as chemical modifiers (formic acid, ammonium acetate and ammonium hydroxide), organic solvent and gradients of the mobile phases in the separation of oligosaccharides have been studied. The best results were achieved on the BEH amide stationary phase, using acetonitrile:water with 0.1% ammonium hydroxide as mobile phase, where the most of oligosaccharides were successfully resolved. Characteristic MS(2) fragmentation profiles of disaccharides containing galactose, glucose and/or fructose units with different linkages were evaluated and used for the characterization of di-, tri- and tetrasaccharides of three commercial prebiotic GOS mixtures (GOS-1, GOS-2 and GOS-3) by HILIC-MS(n). Similar qualitative and quantitative composition was observed for GOS-1 and GOS-3, whereas different linkages and abundances were detected for GOS-2. In general, (1â 4) and (1â 6) glycosidic linkages were the main structures found in GOS, although (1â 2) and (1â 3) linkages were also identified. Regarding molecular weight, up to pentasaccharides were detected in these samples, disaccharides being the most abundant carbohydrates. |
ISSN | 00219673 |
Volume Number | 1220 |
e-ISSN | 18733778 |
Journal | Journal of Chromatography A |
Language | English |
Publisher | Elsevier |
Publisher Date | 2012-01-13 |
Publisher Place | Netherlands |
Access Restriction | Subscribed |
Subject Keyword | Chromatography, Liquid Methods Galactose Analogs & Derivatives Oligosaccharides Analysis Prebiotics Tandem Mass Spectrometry Acetates Acetonitriles Ammonium Hydroxide Carbohydrate Conformation Formates Chemistry Hydrophobic And Hydrophilic Interactions Hydroxides Maltose Molecular Weight Journal Article Research Support, Non-u.s. Gov't Discipline Analytical Chemistry |
Content Type | Text |
Resource Type | Article |
Subject Domain (in MeSH) | Inorganic Chemicals Organic Chemicals Carbohydrates Investigative Techniques Chemical Phenomena |
Subject | Biochemistry Analytical Chemistry Organic Chemistry Medicine |
National Digital Library of India (NDLI) is a virtual repository of learning resources which is not just a repository with search/browse facilities but provides a host of services for the learner community. It is sponsored and mentored by Ministry of Education, Government of India, through its National Mission on Education through Information and Communication Technology (NMEICT). Filtered and federated searching is employed to facilitate focused searching so that learners can find the right resource with least effort and in minimum time. NDLI provides user group-specific services such as Examination Preparatory for School and College students and job aspirants. Services for Researchers and general learners are also provided. NDLI is designed to hold content of any language and provides interface support for 10 most widely used Indian languages. It is built to provide support for all academic levels including researchers and life-long learners, all disciplines, all popular forms of access devices and differently-abled learners. It is designed to enable people to learn and prepare from best practices from all over the world and to facilitate researchers to perform inter-linked exploration from multiple sources. It is developed, operated and maintained from Indian Institute of Technology Kharagpur.
Learn more about this project from here.
NDLI is a conglomeration of freely available or institutionally contributed or donated or publisher managed contents. Almost all these contents are hosted and accessed from respective sources. The responsibility for authenticity, relevance, completeness, accuracy, reliability and suitability of these contents rests with the respective organization and NDLI has no responsibility or liability for these. Every effort is made to keep the NDLI portal up and running smoothly unless there are some unavoidable technical issues.
Ministry of Education, through its National Mission on Education through Information and Communication Technology (NMEICT), has sponsored and funded the National Digital Library of India (NDLI) project.
Sl. | Authority | Responsibilities | Communication Details |
---|---|---|---|
1 | Ministry of Education (GoI), Department of Higher Education |
Sanctioning Authority | https://www.education.gov.in/ict-initiatives |
2 | Indian Institute of Technology Kharagpur | Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project | https://www.iitkgp.ac.in |
3 | National Digital Library of India Office, Indian Institute of Technology Kharagpur | The administrative and infrastructural headquarters of the project | Dr. B. Sutradhar bsutra@ndl.gov.in |
4 | Project PI / Joint PI | Principal Investigator and Joint Principal Investigators of the project |
Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon |
5 | Website/Portal (Helpdesk) | Queries regarding NDLI and its services | support@ndl.gov.in |
6 | Contents and Copyright Issues | Queries related to content curation and copyright issues | content@ndl.gov.in |
7 | National Digital Libarray of India Club (NDLI Club) | Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach | clubsupport@ndl.gov.in |
8 | Digital Preservation Centre (DPC) | Assistance with digitizing and archiving copyright-free printed books | dpc@ndl.gov.in |
9 | IDR Setup or Support | Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops | idr@ndl.gov.in |
Loading...
|