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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Grus, Franz H. Dirk, Desiree R. Joachim, Stephanie C. Wax, Martin B. Boehm, Nils Pfeiffer, Norbert |
Description | Country affiliation: Germany Author Affiliation: Joachim SC ( Center for Vision Science, Ruhr University Eye Hospital, Bochum, Germany.) |
Abstract | PURPOSE: The aim of this study was to characterize the serum antibody reactivities occurring after ocular ischemia reperfusion. The time course of serum antibody responses was examined. METHODS: Wistar rats were exposed to transient ocular ischemia by elevating intraocular pressure to 130 mm Hg for 60 minutes. Axonal damage was evaluated on optic-nerve sections 2 and 4 weeks later. Blood samples collected before and several times after ischemia were used for antibody detection via customized protein microarrays. Different tissue antigens, including heat shock proteins (HSPs) and crystallins, were selected based on previous identification of antibody reactivities in studies on ischemic events or ophthalmic diseases associated with ischemia. Antibody reactivity was compared using multivariate statistical techniques. RESULTS: Significant axonal damage was observed 2 and 4 weeks after ocular ischemia (P < 0.05). Animals showed certain immunoreactivities against antigens even before ischemia, whereas many reactivities increased afterward. Significantly different responses were detected 2, 3, and 4 weeks after ischemia (P < 0.05). Antibody reactivity against actin, glial fibrillary acidic protein, HSP 27, vimentin, or spectrin continually increased. CONCLUSIONS: Ischemia induced by acute intraocular pressure elevation led to complex changes in antibody reactivities in sera of treated animals. Upregulation of serum autoantibodies, especially against heat shock and structural proteins, progressively increased throughout the 4-week follow-up period, whereas others such as ubiquitin decreased. The upregulation of anti-HSP 27 antibodies might be an attempt to protect the tissue from ischemic damage. |
ISSN | 01460404 |
e-ISSN | 15525783 |
Journal | Investigative Opthalmology & Visual Science |
Issue Number | 6 |
Volume Number | 52 |
Language | English |
Publisher | Association for Research in Vision and Ophthalmology |
Publisher Date | 2011-06-01 |
Publisher Place | United States |
Access Restriction | Open |
Subject Keyword | Autoantibodies Blood Autoantigens Immunology Eye Proteins Hsp27 Heat-shock Proteins Reperfusion Injury Retinal Diseases Animals Axons Pathology Glial Fibrillary Acidic Protein Immunoglobulin G Intraocular Pressure Myelin Basic Protein Myelin Proteins Myelin-associated Glycoprotein Myelin-oligodendrocyte Glycoprotein Optic Nerve Protein Array Analysis Rats, Wistar Retinal Vessels Spectrin Up-regulation Research Support, Non-u.s. Gov't Discipline Ophthalmology |
Content Type | Text |
Resource Type | Article |
Subject | Cellular and Molecular Neuroscience Ophthalmology Sensory Systems |
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