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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Marasso, Simone Luigi Ferrante, Ivan Frascella, Francesca Santoro, Karin Castagna, Riccardo Ricciardi, Carlo Gili, Marilena Pezzolato, Marzia Bozzetta, Elena Pitardi, Danilo |
Description | Country affiliation: Italy Author Affiliation: Ricciardi C ( Politecnico di Torino-LATEMAR Unit, Department of Applied Science and Technology, Corso Duca degli Abruzzi 24, I-10129 Torino, Italy. carlo.ricciardi@polito.it) |
Abstract | To date control strategies in detecting anabolic agents for promoting growth of food producing animals are mainly related to screening techniques based on immunochemical and physiochemical methods, whose major limit is represented by relative low analytical sensitivity. As a consequence, consumers are currently exposed to molecules with potential carcinogenic effects such as 17ß-estradiol, the most powerful substance with estrogenic effect. Therefore, high analytical sensitivity screening and confirmatory methods are required, coupling easiness of use and efficiency. We here report on the immunodetection of 17ß-estradiol in serum by antibody-immobilized microcantilever resonators, an innovative biosensing platform able to quantify an adsorbed target mass (such as cells, nucleic acids, biomolecules, etc.) thanks to a shift in resonance frequency. Our tool based on microcantilever resonator arrays has shown to be capable of discriminating treated and untreated animals, showing the ability of detecting traces of 17ß-estradiol in serum at concentrations lower than the present accepted physiological serum concentration threshold value (40 ppt) and commercial ELISA tests (25 ppt). The method exhibits a limit of detection of 20 ppt and a limited cross-reactivity with high concentrations (10 ppb) of similar molecules (testosterone). |
ISSN | 09565663 |
Issue Number | 1 |
Volume Number | 40 |
e-ISSN | 18734235 |
Journal | Biosensors and Bioelectronics |
Language | English |
Publisher | Elsevier |
Publisher Date | 2013-02-15 |
Publisher Place | Great Britain (UK) |
Access Restriction | Subscribed |
Subject Keyword | Biosensing Techniques Instrumentation Estradiol Blood Immunoassay Micro-electrical-mechanical Systems Microchemistry Equipment Design Equipment Failure Analysis Reproducibility Of Results Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't Discipline Biotechnology |
Content Type | Text |
Resource Type | Article |
Subject Domain (in MeSH) | Polycyclic Compounds Investigative Techniques Equipment and Supplies |
Subject | Nanoscience and Nanotechnology Biomedical Engineering Biophysics Electrochemistry Biotechnology Medicine |
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