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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Tanke, Hans J. Molenaar, Chris Gena, Aarti Abdulle, Abadir Dirks, Roeland W. |
Description | Author Affiliation: Molenaar C ( Dept. of Molecular Cell Biology, Leiden University Medical Center, Wassenaarseweg 72, 2333 AL Leiden, Netherlands.) |
Abstract | Many of the protein factors that play a role in nuclear export of mRNAs have been identified, but still little is known about how mRNAs are transported through the cell nucleus and which nuclear compartments are involved in mRNA transport. Using fluorescent 2'O-methyl oligoribonucleotide probes, we investigated the mobility of $poly(A)^{+}$ RNA in the nucleoplasm and in nuclear speckles of U2OS cells. Quantitative analysis of diffusion using photobleaching techniques revealed that the majority of $poly(A)^{+}$ RNA move throughout the nucleus, including in and out of speckles (also called SC-35 domains), which are enriched for splicing factors. Interestingly, in the presence of the transcription inhibitor 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole, the association of $poly(A)^{+}$ RNA with speckles remained dynamic. Our results show that RNA movement is energy dependent and that the proportion of nuclear $poly(A)^{+}$ RNA that resides in speckles is a dynamic population that transiently interacts with speckles independent of the transcriptional status of the cell. Rather than the $poly(A)^{+}$ RNA within speckles serving a stable structural role, our findings support the suggestion of a more active role of these regions in nuclear RNA metabolism and/or transport. |
ISSN | 00219525 |
e-ISSN | 15408140 |
Journal | The Journal of Cell Biology |
Issue Number | 2 |
Volume Number | 165 |
Language | English |
Publisher | Rockefeller University Press (United States) |
Publisher Date | 2004-04-26 |
Publisher Place | United States |
Access Restriction | Open |
Subject Keyword | Cell Nucleus Metabolism Fluorescent Dyes RNA, Messenger Transcription, Genetic Active Transport, Cell Nucleus Physiology Cell Line Cell Line, Tumor Deoxyadenosines Dichlororibofuranosylbenzimidazole Fluorescence Recovery After Photobleaching Nuclear Proteins Nucleic Acid Synthesis Inhibitors Oligoribonucleotides Chemistry Poly(A)-Binding Protein II RNA-Binding Proteins Recombinant Fusion Proteins Rhodamines Serine-Arginine Splicing Factors Research Support, Non-U.S. Gov't Cell Biology |
Content Type | Text |
Resource Type | Article |
Subject | Cell Biology Medicine |
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