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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Dietrich, A. Sakamoto, W. Wintz, H. Maréchal-drouard, L. Menand, B. |
Description | Author Affiliation: Menand B ( Institut de Biologie Moléculaire des Plantes/Centre National de la Recherche Scientifique, Université Louis Pasteur, 12 rue du Général Zimmer, F-67084 Strasbourg Cedex, France.); |
Abstract | One-fifth of the tRNAs used in plant mitochondrial translation is coded for by chloroplast-derived tRNA genes. To understand how aminoacyl-tRNA synthetases have adapted to the presence of these tRNAs in mitochondria, we have cloned an Arabidopsis thaliana cDNA coding for a methionyl-tRNA synthetase. This enzyme was chosen because chloroplast-like elongator tRNAMet genes have been described in several plant species, including A. thaliana. We demonstrate here that the isolated cDNA codes for both the chloroplastic and the mitochondrial methionyl-tRNA synthetase (MetRS). The protein is transported into isolated chloroplasts and mitochondria and is processed to its mature form in both organelles. Transient expression assays using the green fluorescent protein demonstrated that the N-terminal region of the MetRS is sufficient to address the protein to both chloroplasts and mitochondria. Moreover, characterization of MetRS activities from mitochondria and chloroplasts of pea showed that only one MetRS activity exists in each organelle and that both are indistinguishable by their behavior on ion exchange and hydrophobic chromatographies. The high degree of sequence similarity between A. thaliana and Synechocystis MetRS strongly suggests that the A. thaliana MetRS gene described here is of chloroplast origin. |
ISSN | 00278424 |
e-ISSN | 10916490 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Issue Number | 18 |
Volume Number | 95 |
Language | English |
Publisher | National Academy of Sciences |
Publisher Date | 1998-09-01 |
Publisher Place | United States |
Access Restriction | Open |
Subject Keyword | Arabidopsis Genetics Chloroplasts Enzymology Methionine-tRNA Ligase Mitochondria Amino Acid Sequence Biological Transport DNA, Complementary Green Fluorescent Proteins Luminescent Proteins Isolation & Purification Metabolism Molecular Sequence Data Research Support, Non-U.S. Gov't Multidisciplinary |
Content Type | Text |
Resource Type | Article |
Subject | Multidisciplinary |
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