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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Di Serio, F. Bouldoires, E. Schob, H. Meins, F. Iglesias, A. Tarina, C. |
Description | Author Affiliation: Di Serio F ( Friedrich Miescher Institute, a branch of the Novartis Research Foundation, Maulbeerstrasse 66, CH-4058 Basel, Switzerland.); |
Abstract | Antisense-mediated gene silencing (ASGS) and posttranscriptional gene silencing (PTGS) with sense transgenes markedly reduce the steady-state mRNA levels of endogenous genes similar in transcribed sequence. RNase protection assays established that silencing in tobacco plants transformed with plant-defense-related class I sense and antisense chitinase (CHN) transgenes is at the posttranscriptional level. Infection of tobacco plants with cucumber mosaic virus strain FN and a necrotizing strain of potato virus Y, but not with potato virus X, effectively suppressed PTGS and ASGS of both the transgenes and homologous endogenes. This suggests that ASGS and PTGS share components associated with initiation and maintenance of the silent state. Small, ca. 25-nt RNAs (smRNA) of both polarities were associated with PTGS and ASGS in CHN transformants as reported for PTGS in other transgenic plants and for RNA interference in Drosophila. Similar results were obtained with an antisense class I beta-1,3-glucanase transformant showing that viral suppression and smRNAs are a more general feature of ASGS. Several current models hold that diverse signals lead to production of double-stranded RNAs, which are processed to smRNAs that then trigger PTGS. Our results provide direct evidence for mechanistic links between ASGS and PTGS and suggest that ASGS could join a common PTGS pathway at the double-stranded RNA step. |
ISSN | 00278424 |
e-ISSN | 10916490 |
Journal | Proceedings of the National Academy of Sciences of the United States of America |
Issue Number | 11 |
Volume Number | 98 |
Language | English |
Publisher | National Academy of Sciences |
Publisher Date | 2001-05-01 |
Publisher Place | United States |
Access Restriction | Open |
Subject Keyword | Chitinase Genetics Cucumovirus Physiology Gene Expression Regulation, Enzymologic Gene Expression Regulation, Plant Gene Silencing Plants, Toxic Potyvirus RNA Processing, Post-Transcriptional Tobacco Beta-Glucosidase Metabolism Glucan 1,3-beta-Glucosidase Plant Proteins RNA, Antisense RNA, Plant Transformation, Genetic Multidisciplinary |
Content Type | Text |
Resource Type | Article |
Subject | Multidisciplinary |
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