NDLI: The influence of semen-derived enhancer of virus infection on the efficiency of retroviral gene transfer.

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Year: 2010 Volume: 12 Issue: 1

Year: 2009 Volume: 11

Year: 2008 Volume: 10

The influence of semen-derived enhancer of virus infection on the efficiency of retroviral gene transfer.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wurm, Melanie Schambach, Axel Lindemann, Dirk Hanenberg, Helmut Ständker, Ludger Forssmann, Wolf-Georg Blasczyk, Rainer Horn, Peter A.
Description	Country affiliation: Germany Author Affiliation: Wurm M ( Institute for Transfusion Medicine, Hannover Medical School, Hannover, Germany.)
Abstract	BACKGROUND: An improvement of retroviral infection has been postulated using a naturally occurring fragment of the abundant semen marker prostatic acidic phosphatase. This peptide, termed semen-derived enhancer of virus infection (SEVI), promotes HIV attachment to the target cells. METHODS: In the present study, we examined whether SEVI would also enhance the infectivity of other viruses with different envelope proteins. We focused on retroviruses pseudotyped with envelopes that are commonly used for the genetic modification of cells, in particular, T cells and hematopoietic progenitor cells. Because the effect of SEVI is considered to be a result of its cationic properties, we compared SEVI with other cationic agents such as protamine sulfate and Polybrene. RESULTS: We found that SEVI increases the efficiency of gene transfer for lentiviral and gammaretroviral vector constructs pseudotyped with VSV-G, GALV, RD114 or foamy viral envelopes on hematopoietic and nonhematopoietic cell lines. On T cells, the transduction efficiency of GALV and RD114 pseudotyped vectors was significantly increased by SEVI. A significant increase of the gene transfer rate was also detected for foamy virally pseudotyped lentivirus on murine hematopoietic progenitor cells. No toxic effect of SEVI treatment was detected on any cell type tested, including human and murine hematopoietic stem/progenitor cells. When directly comparing the effect of SEVI with Polybrene or protamine sulfate, we show that the semen-derived protein is more efficient in increasing the gene transfer rate. CONCLUSIONS: SEVI is a promising agent for promoting and improving gene transfer and may also be useful for clinical gene therapy studies.
File Format	HTM / HTML
ISSN	1099498X
Issue Number	2
Volume Number	12
e-ISSN	15212254
Journal	The Journal of Gene Medicine
Language	English
Publisher	Wiley
Publisher Date	2010-02-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Molecular Biology Gene Transfer Techniques Peptide Fragments Pharmacology Protein Tyrosine Phosphatases Retroviridae Genetics Acid Phosphatase Animals Antigens, Cd34 Metabolism Cations Cell Adhesion Drug Effects Cell Death Flow Cytometry Hela Cells Hematopoietic System Cytology Humans Male Mice Nih 3t3 Cells Chemistry Transduction, Genetic Viral Envelope Proteins Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject Domain (in MeSH)	Cells Hemic and Immune Systems Eukaryota Viruses Inorganic Chemicals Enzymes and Coenzymes Amino Acids, Peptides, and Proteins Biological Factors Diagnosis Investigative Techniques Cell Physiological Phenomena
Subject	Molecular Biology Drug Discovery Genetics Molecular Medicine Genetics (clinical)

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