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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Lo, Joe Fu-Jiou Wang, Yong Li, Zidong Zhao, Zhengtuo Hu, Di Eddington, David T. Oberholzer, Jose |
Description | Author Affiliation: Lo JF ( Department of Mechanical Engineering, University of Michigan-Dearborn.) |
Abstract | Simultaneous oxygenation and monitoring of glucose stimulus-secretion coupling factors in a single technique is critical for modeling pathophysiological states of islet hypoxia, especially in transplant environments. Standard hypoxic chamber techniques cannot modulate both stimulations at the same time nor provide real-time monitoring of glucose stimulus-secretion coupling factors. To address these difficulties, we applied a multilayered microfluidic technique to integrate both aqueous and gas phase modulations via a diffusion membrane. This creates a stimulation sandwich around the microscaled islets within the transparent polydimethylsiloxane (PDMS) device, enabling monitoring of the aforementioned coupling factors via fluorescence microscopy. Additionally, the gas input is controlled by a pair of microdispensers, providing quantitative, sub-minute modulations of oxygen between 0-21%. This intermittent hypoxia is applied to investigate a new phenomenon of islet preconditioning. Moreover, armed with multimodal microscopy, we were able to look at detailed calcium and KATP channel dynamics during these hypoxic events. We envision microfluidic hypoxia, especially this simultaneous dual phase technique, as a valuable tool in studying islets as well as many ex vivo tissues. |
File Format | HTM / HTML |
e-ISSN | 1940087X |
DOI | 10.3791/50616 |
Journal | Journal of Visualized Experiments |
Issue Number | 81 |
Language | English |
Publisher | MyJove Corp. |
Publisher Date | 2013-11-17 |
Publisher Place | United States |
Access Restriction | Open |
Subject Keyword | Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Islets Of Langerhans Cytology Drug Effects Microfluidic Analytical Techniques Instrumentation Oxygen Administration & Dosage Animals Cell Hypoxia Physiology Glucose Metabolism Mice Mice, Inbred C57bl Microscopy, Fluorescence Single-cell Analysis Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s. Video-audio Media |
Content Type | Text |
Resource Type | Article |
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