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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Cavada, Benildo S. Marinho, Emmanuel S. Souza, Emmanuel P. Benevides, Raquel G. Delatorre, Plínio Souza, Luis A. G. Nascimento, Kyria S. Sampaio, Alexandre H. Moreno, Frederico B. M. B. Rustiguel, Joane K. R. Canduri, Fernanda de Azevedo, Walter F. Debray, Henri |
Description | Country affiliation: Brazil Author Affiliation: Cavada BS ( Department of Biochemistry, Federal University of Ceará, Brazil. bscavada@ufc.br) |
Abstract | A lectin from Cymbosema roseum seeds (CRL) was purified, characterized and crystallized. The best crystals grew in a month and were obtained by the vapour-diffusion method using a precipitant solution consisting of 0.1 M Tris-HCl pH 7.8, 8%(w/v) PEG 3350 and 0.2 M proline at a constant temperature of 293 K. A data set was collected to 1.77 A resolution at a synchrotron-radiation source. CRL crystals are orthorhombic, belonging to space group P2(1)2(1)2(1). Crystallographic refinement and full amino-acid sequence determination are in progress. |
File Format | HTM / HTML |
ISSN | 2053230X |
e-ISSN | 17443091 |
Journal | Acta Crystallographica Section F Structural Biology and Crystallization Communications |
Issue Number | Pt 3 |
Volume Number | 62 |
Language | English |
Publisher | Wiley |
Publisher Date | 2006-03-01 |
Publisher Place | Great Britain (UK) |
Access Restriction | Open |
Subject Keyword | Discipline Crystallography Discipline Biophysics Discipline Molecular Biology Discipline Biochemistry Fabaceae Chemistry Plant Lectins Isolation & Purification Amino Acid Sequence Animals Chromatography, Affinity Crystallization Crystallography, X-ray Hemagglutination Mannose Molecular Sequence Data Pharmacology Rabbits Research Support, Non-u.s. Gov't |
Content Type | Text |
Resource Type | Article |
Subject | Condensed Matter Physics Biochemistry Biophysics Structural Biology Genetics Medicine |
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