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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Michaud, François-Thomas Havugimana, Pierre Claver Duchesne, Carl Sanschagrin, François Bernier, Alice Lévesque, Roger C. Garnier, Alain |
Description | Author Affiliation: Michaud FT ( Feldan Inc., 4975 Rideau Street, Québec, QC, Canada G2E 5H5.) |
Abstract | Liquid chromatography mass spectrometry (LCMS) is a powerful technique that could serve to rapidly characterize cell culture protein expression profile and be used as a process monitoring and control tool. However, this application is often hampered by both the sample proteome and the LCMS signal complexities as well as the variability of this signal. To alleviate this problem, culture samples are usually extensively fractionated and pretreated before being analyzed by top-end instruments. Such an approach precludes LCMS usage for routine on-line or at-line application. In this work, by applying multivariate analysis (MA) directly on raw LCMS signals, we were able to extract relevant information from cell culture samples that were simply lyzed. By using the recombinant adenovirus production process as a model, we were able to follow the accumulation of the three major proteins produced, identified their accumulation dynamics, and draw useful conclusions from these results. The combination of LCMS and MA provides a simple, rapid, and precise means to monitor cell culture. |
File Format | HTM / HTML |
ISSN | 02732289 |
Issue Number | 3 |
Volume Number | 167 |
e-ISSN | 15590291 |
Journal | Applied Biochemistry and Biotechnology |
Language | English |
Publisher | Springer |
Publisher Date | 2012-06-01 |
Publisher Place | United States |
Access Restriction | Subscribed |
Subject Keyword | Discipline Biochemistry Discipline Biotechnology Cell Culture Techniques Methods Chromatography, Liquid Mass Spectrometry Adenoviridae Genetics Centrifugation Dna, Recombinant Hek293 Cells Humans Multivariate Analysis Software Solubility Time Factors Virion Metabolism Journal Article Research Support, Non-u.s. Gov't |
Content Type | Text |
Resource Type | Article |
Subject Domain (in MeSH) | Cells Viral Structures Eukaryota Viruses Nucleic Acids, Nucleotides, and Nucleosides Diagnosis Investigative Techniques Physical Phenomena Chemical Phenomena Information Science |
Subject | Biotechnology Applied Microbiology and Biotechnology Biochemistry Environmental Engineering Bioengineering Molecular Biology Medicine |
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