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Content Provider | World Health Organization (WHO)-Global Index Medicus |
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Author | Hu, Haobin Liang, Haipeng Wu, Yun |
Description | Author Affiliation: Hu H ( College of Chemistry & Chemical Engineering, Longdong University, Qingyang 745000, PR China. Electronic address: hhb-88@126.com.); Liang H ( Department of Oncology, Qingyang First People's Hospital, Qingyang 745000, PR China.); Wu Y ( College of Chemistry & Chemical Engineering, Longdong University, Qingyang 745000, PR China.) |
Abstract | A water-soluble polysaccharide, designated as ABPS-21, was isolated from the stem barks of Acanthopanax brachypus by hot-water extraction and purified by Cellulose DEAE-52 and Sephacryl S-300 HR gel-filtration chromatography, respectively. The homogeneity and molecular weight were determined using HPGPC. The structure was elucidated based on monosaccharide composition and methylation analysis, partial acid hydrolysis, periodate oxidation, Smith degradation, IR and NMR spectroscopy. The result showed that ABPS-21 was a homogeneous heteropolysaccharide including galactose, glucose, rhamnose and galacturonic acid in a molar ratio of 3.0:2.0:2.0:1.0, respectively, with an average molecular weight of 1.06 × 10(5)Da. The main chain of ABPS-21 was made up of â 4)-ß-D-Galp-(1 â 4)-ß-D-Glcp-(1 â 4)-ß-D-Galp-(1 â 4)-ß-D-Galp-(1 â 2)- -L-Rhap-(1 â, and two side chains -l-Rhap-(1 â 4)-ß-D-Glcp-(1 â and -D-galpA-(1 â were attached to the backbone chain at O-6 position of 1,4,6-linked ß-D-Glcp and 1,4,6-linked ß-D-Galp, respectively. ABPS-21, an acidic branched polysaccharide from A. brachypus, was not described up to now. |
File Format | HTM / HTML |
ISSN | 01448617 |
Volume Number | 127 |
e-ISSN | 18791344 |
Journal | Carbohydrate Polymers |
Language | English |
Publisher | Elsevier |
Publisher Date | 2015-01-01 |
Publisher Place | Great Britain (UK) |
Access Restriction | Subscribed |
Subject Keyword | Discipline Biochemistry__semicolon__materials Discipline Science Eleutherococcus Chemistry Polysaccharides Carbohydrate Sequence Galactose Analysis Glucose Hexuronic Acids Molecular Sequence Data Plant Stems Rhamnose Journal Article Research Support, Non-u.s. Gov't |
Content Type | Text |
Resource Type | Article |
Subject Domain (in MeSH) | Plant Structures Eukaryota Organic Chemicals Carbohydrates Chemical Phenomena Information Science |
Subject | Polymers and Plastics Materials Chemistry Organic Chemistry |
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