NDLI: Filtering technique for fluorescence-based DNA sequencing data

Content Provider	IEEE Xplore Digital Library
Author	Domnisoru, C. Musavi, M.
Copyright Year	2000
Description	Author affiliation: Maine Univ., Orono, ME, USA (Domnisoru, C.)
Abstract	An important aspect in data processing for the four dye fluorescence-based DNA sequencing is the crosstalk filtering. Typically, a matrix M, which is function on the fluorophores employed and the fluorescence detection system, is used in the multicomponent analysis. In the deconvolution process the matrix is applied directly to the raw signal, on a linear crosstalk assumption. This necessitates the signal to be aligned to the baseline. The various techniques used for aligning the raw data have as negative effect an additional distortion of the signal. An algorithm for crosstalk removal is presented. It is based on using the variation of the raw signal (instead of the signal itself) thus making possible the crosstalk removal before the base line adjustment. In addition, a supplementary filtering step is proposed in order to account for the fact that the crosstalk is in reality nonlinear. This second step is based on a matrix T and accounts for the influence on each of the signals from the derivatives of the other three. The overall result is less information lost through filtering. Our strategy helps in preserving the information contained in the raw data. Consequently, we consider the data processed using our proposed algorithm can allow for a better accuracy in base calling.
Starting Page	489
Ending Page	493
File Size	407987
Page Count	5
File Format	PDF
ISBN	0780359577
ISSN	08407789
DOI	10.1109/CCECE.2000.849757
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2000-05-07
Publisher Place	Canada
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fluorescence DNA Time measurement Wavelength measurement Data processing Deconvolution Signal processing Nonlinear distortion Information filtering Information filters
Content Type	Text
Resource Type	Article
Subject	Electrical and Electronic Engineering Hardware and Architecture

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Mr. Siddhartha Mukherjee siddhartha@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	ndl-support@iitkgp.ac.in
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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