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Cell Culturing Method and Cell Culturing System
| Content Provider | The Lens |
|---|---|
| Description | Procédé et système de culture cellulaire (10). Le procédé de culture de cellules comprend une étape de mesure et une étape pour déterminer la culture. Dans l'étape de mesure, une concentration d'acide glutamique d'un milieu de culture collecté est mesurée. Dans l'étape permettant de déterminer la culture, on détermine si un état de culture cellulaire se trouve ou non au stade final d'une phase de croissance logarithmique, sur la base d'une concentration d'acide glutamique mesurée, à savoir la concentration d'acide glutamique ayant été mesurée. Le système de culture cellulaire (10) comprend un trajet d'écoulement d'échantillonnage (98), un biocapteur (90) et une unité de détermination de culture (162). |
| Abstract | The present invention relates to a cell culturing method and a cell culturing system (10). The cell culturing method includes a measurement step and a culture determination step. In the measurement step, a glutamic acid concentration of a collected culture medium is measured. In the culture determination step, a determination is made as to whether or not a cell culturing state is in a final stage of a logarithmic growth phase, based on a measured glutamic acid concentration, which is the glutamic acid concentration that was measured. The cell culturing system (10) includes a sampling flow path (98), a biosensor (90), and a culture determination unit (162). |
| Related Links | https://www.lens.org/images/patent/WO/2023054080/A1/WO_2023_054080_A1.pdf |
| Language | English |
| Publisher Date | 2023-04-06 |
| Access Restriction | Open |
| Alternative Title | Procédé Et Système De Culture Cellulaire |
| Content Type | Text |
| Resource Type | Patent |
| Date Applied | 2022-09-21 |
| Agent | Chiba Yoshihiro Et Al. |
| Applicant | Terumo Corp |
| Application No. | 2022035071 |
| Claim | A cell culturing method, comprising: a measurement step of measuring a glutamic acid concentration of a culture medium used in performing culturing of the cells; and a culture determination step of determining whether or not a cell culturing state is in a final stage of a logarithmic growth phase, based on a measured glutamic acid concentration, which is the glutamic acid concentration that was measured. The cell culturing method according to claim 1, wherein, in the culture determination step, in the case that the glutamic acid concentration has exceeded a threshold value, a determination is made that the cell culturing state is in the final stage of the logarithmic growth phase, whereas, in the case that the glutamic acid concentration is less than or equal to the threshold value, a determination is made that the cell culturing state is not in the final stage of the logarithmic growth phase. The cell culturing method according to claim 1, wherein, in the culture determination step, in the case that an absolute value of a rate of change in the measured glutamic acid concentration has exceeded a reference rate of change, a determination is made that the cell culturing state is in the final stage of the logarithmic growth phase, whereas, in the case that the absolute value of the rate of change in the measured glutamic acid concentration is less than or equal to the reference rate of change, a determination is made that the cell culturing state is not in the final stage of the logarithmic growth phase. The cell culturing method according to claim 1, wherein, in the culture determination step, in the case that immediately after the measured glutamic acid concentration has risen so that the rate of change in the measured glutamic acid concentration exceeds a first reference rate of change, the measured glutamic acid concentration has descended so that the absolute value of the rate of change in the measured glutamic acid concentration exceeds a second reference rate of change, a determination is made that the cell culturing state is in the final stage of the logarithmic growth phase, whereas, in the case that the rate of change is less than or equal to the first reference rate of change when the measured glutamic acid concentration rises, or is less than or equal to the absolute value of the second rate of change when the measured glutamic acid concentration descends, a determination is made that the cell culturing state is not in the final stage of the logarithmic growth phase. The cell culturing method according to any one of claims 1 to 4, further comprising a notification step of issuing a notification of predetermined information, in the case that a determination is made in the culture determination step that the cell culturing state is in the final stage of the logarithmic growth phase. The cell culturing method according to any one of claims 1 to 5, wherein the cells are cultured inside a bioreactor. The cell culturing method according to claim 6, further comprising a collection step of collecting the cells inside the bioreactor, in the case that a determination is made in the culture determination step that the cell culturing state is in the final stage of the logarithmic growth phase. The cell culturing method according to claim 7, wherein, in the collection step, the cells inside the bioreactor are guided to a collection container by a pump installed in a flow path connected to the bioreactor. The cell culturing method according to any one of claims 1 to 8, further comprising a sampling step of obtaining the culture medium. A cell culturing system configured to perform culturing of the cells, the cell culturing system comprising: a sampling flow path configured to obtain a culture medium used in performing culturing of the cells; a biosensor installed in the sampling flow path, and configured to measure a glutamic acid concentration of the culture medium flowing through the sampling flow path; and a culture determination unit configured to determine whether or not a cell culturing state is in a final stage of a logarithmic growth phase, based on a measured glutamic acid concentration, which is the glutamic acid concentration that was measured. The cell culturing system according to claim 10, wherein, in the case that the glutamic acid concentration has exceeded a threshold value, the culture determination unit determines that the cell culturing state is in the final stage of the logarithmic growth phase, whereas, in the case that the glutamic acid concentration is less than or equal to the threshold value, the culture determination unit determines that the cell culturing state is not in the final stage of the logarithmic growth phase. The cell culturing system according to claim 10, wherein, in the case that an absolute value of a rate of change in the measured glutamic acid concentration has exceeded a reference rate of change, the culture determination unit determines that the cell culturing state is in the final stage of the logarithmic growth phase, whereas, in the case that the absolute value of the rate of change in the measured glutamic acid concentration is less than or equal to the reference rate of change, the culture determination unit determines that the cell culturing state is not in the final stage of the logarithmic growth phase. The cell culturing system according to claim 10, wherein in the case that immediately after the measured glutamic acid concentration has risen so that the rate of change in the measured glutamic acid concentration exceeds a first reference rate of change, the measured glutamic acid concentration has descended so that the absolute value of the rate of change in the measured glutamic acid concentration exceeds a second reference rate of change, the culture determination unit determines that the cell culturing state is in the final stage of the logarithmic growth phase, whereas, in the case that the rate of change is less than or equal to the first reference rate of change when the measured glutamic acid concentration rises, or is less than or equal to the absolute value of the second rate of change when the measured glutamic acid concentration descends, the culture determination unit determines that the cell culturing state is not in the final stage of the logarithmic growth phase. The cell culturing system according to any one of claims 10 to 13, further comprising a notification control unit configured to control a notification unit to issue a predetermined notification from the notification unit, in the case that a determination is made by the culture determination unit that the cell culturing state is in the final stage of the logarithmic growth phase. The cell culturing system according to any one of claims 10 to 14, further comprising a bioreactor configured to culture the cells. The cell culturing system according to claim 15, further comprising: a culturing circuit connected to the bioreactor; a collection container connected to the culturing circuit; a pump configured to guide the cells from the bioreactor to the collection container via the culturing circuit by imparting fluidity to a liquid inside the culturing circuit; a clamp configured to open and close a collection flow path forming at least a portion of a pathway from the bioreactor to the collection container in the culturing circuit; a pump control unit configured to control the pump; and a clamp control unit configured to control the clamp; wherein, in the case it is determined by the culture determination unit that the cell culturing state is in the final stage of the logarithmic growth phase, the clamp control unit opens the clamp to thereby cause the collection flow path to open, together with the pump control unit controlling the pump to guide the cells from the bioreactor to the collection container via the culturing circuit. |
| CPC Classification | APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY;APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS; FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS; i.e. BIOREACTORS OR FERMENTERS |
| Extended Family | 102-612-195-411-681 |
| Patent ID | 2023054080 |
| Inventor/Author | Igarashi Masatsugu Hirahara Ichirou |
| IPC | C12M1/34 C12M1/36 |
| Status | Pending |
| Simple Family | 102-612-195-411-681 |
| CPC (with Group) | C12M41/32 C12M41/48 |
| Issuing Authority | World Intellectual Property Organization (WIPO) |
| Kind | Patent Application Publication |
