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Author Krishnan, L. K. ♦ Sulochana, P. V. ♦ Mathai, J. ♦ John, Annie ♦ Sivakumar, R.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Indian Journal of Medical Research
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health ♦ Diseases
Subject Domain (in MeSH) Hemic and Immune Systems ♦ Anatomy
Subject Keyword Hematology
Abstract To assess the effect of storage bags on platelets, we studied the morphological and ultrastructural changes of samples drawn from platelet concentrates (PC) prepared and stored in triple, poly vinyl chloride (PVC) bags, manufactured in India. Using the scanning electron microscopy, we demonstrate formation of long pseudopods, and interaction through these to form aggregates. When platelets were stored at 23+/-2 degrees C, morphological changes were severe compared to the deleterious effects when kept at 22+/-0.5 degrees C. Ultrastructural analysis also showed that maintenance of discoid shape and prevention of granule secretion could be improved by storing the platelets at 22+/-0.5 degrees C. Significant degree of platelet fragmentation took place when the storage temperature was high. The morphology score done for platelets stored st both 22+/-0.5 degrees C and 23+/-2 degrees C showed that preservation of discoid shape was better with the former.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Journal INDIAN JOURNAL OF MEDICAL RESEARCH
Volume Number 105
Page Count 8
Starting Page 77
Ending Page 84