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Author Renjith, R. S. ♦ Jayakumari, N.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Indian Journal of Clinical Biochemistry : Ijcb
File Format PDF
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Technology ♦ Medicine & health
Subject Domain (in MeSH) Lipids ♦ Chemicals and Drugs ♦ Chemical Phenomena ♦ Biological Sciences
Subject Keyword Biochemistry
Abstract In the present study, we report a simple and economical precipitation method for the quantitative determination of small, dense LDL-cholesterol (sdLDL-C) in serum that is considered to be an emerging risk factor for cardiovascular disease. This method consisted of precipitation of lipoproteins of density <1.044g/ml using heparin-MnCl(2) and quantification of cholesterol existed in the supernatant using reagents for routine cholesterol assay instead of the costly direct low density lipoprotein-cholesterol assay kit. The supernatant contained sdLDL and high-density lipoprotein (HDL) that was confirmed by polyacrylamide gel electrophoresis. sdLDL-C concentration can be calculated by subtracting the HDL-C value from the total cholesterol concentration of the supernatant. sdLDL-C values obtained by this modified method were similar to those obtained by direct assay of sdLDL-C and there was significant correlation between the two methods. In conclusion, this method is highly economical, do not require special equipments and is useful to evaluate atherogenic risk.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Journal Indian journal of clinical biochemistry : IJCB
Volume Number 26
Issue Number 4
Page Count 378
Starting Page 8
Ending Page 385