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Author Suboj, Priya ♦ Babykutty, Suboj ♦ Gopi, Deepak Roshan Valiyaparambil ♦ Nair, Rakesh S. ♦ Srinivas, P. ♦ Gopala, S.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher European Journal of Pharmaceutical Sciences
File Format PDF
Language English
Subject Domain (in DDC) Natural sciences & mathematics ♦ Life sciences; biology ♦ Physiology & related subjects ♦ Biochemistry ♦ Technology ♦ Medicine & health
Subject Domain (in MeSH) Neoplasms ♦ Diseases ♦ Polycyclic Compounds ♦ Enzymes and Coenzymes ♦ Amino Acids, Peptides, and Proteins ♦ Chemicals and Drugs ♦ Cell Physiological Phenomena ♦ Physiological Phenomena ♦ Biological Sciences
Subject Keyword Biochemistry
Abstract Aloe emodin (AE), a natural anthraquinone, is reported to have antiproliferative activity in various cancer cell lines. In this study we analyzed molecular mechanisms involved in the antimigratory and antiangiogenic activity of this hydroxy anthraquinone in colon cancer cell, WiDr. Our results show that a relatively non toxic concentration of AE suppressed the phorbol-12-myristyl-13-acetate (PMA) induced migration and invasion of tumor cells. On analysis for the molecules involved in the migration/invasion, we found AE downregulated mRNA expression and promoter/gelatinolytic activity of Matrix Metalloproteinase (MMP)-2/9, as well as the RhoB expression at gene and protein level. It was also a strong inhibitor of Vascular Endothelial Growth Factor (VEGF) expression, promoter activity and endothelial cell migration/invasion and in vitro angiogenesis. AE suppressed the nuclear translocation and DNA binding of NF-kappa B, which is an important transcription factor for controlling MMP-2/9 and VEGF gene expression. Taken together these data indicate that AE target multiple molecules responsible for cellular invasion, migration and angiogenesis. Inhibitory effect on angiogenic and metastatic regulatory processes make AE a sensible candidate as a specific blocker of tumor associated events. (C) 2011 Elsevier B.V. All rights reserved.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Journal EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
Volume Number 45
Issue Number 5
Page Count 11
Starting Page 581
Ending Page 591