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Author Venugopal, Balu ♦ Fernandez, F. B. ♦ Babu, Suresh S. ♦ Harikrishnan, V. S. ♦ Varma, H. K. ♦ John, Annie
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Journal of Biomedical Materials Research Part A
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health ♦ Human physiology
Subject Domain (in MeSH) Cells ♦ Anatomy ♦ Biomedical and Dental Materials ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Physiological Phenomena ♦ Biological Sciences
Subject Keyword Tissue Engineering
Abstract Developing adipose tissue-engineered construct to mend soft tissue defects arising from traumatic injury, tumor resections, and maxillofacial abnormalities is of prime importance in plastic and reconstructive surgical procedures. It is apparent that the clinical outcome of classic techniques like adipose tissue transplantation is unpredictable, with graft resorption, lack of vascularization, and impaired functionality. In this prospective, the concept of tissue engineering was adopted to fabricate a combination product with biphasic calcium phosphate (BCP) and rat adipose-derived mesenchymal stem cells (ASCs) toward the development of an adipose tissue construct. BCP, a combination of hydroxyapatite and a-tricalcium phosphate, was characterized for its physiochemical properties, and ASCs were characterized for their stemness. The cellceramic interactions were demonstrated in vitro, whereas adipogenesis was picturesquely depicted by Nile red-stained multilocular adipocyte-like cells. Subsequently, the three-dimensional cellceramic-engineered construct was implanted in the rat dorsal muscle for a period of 3 weeks to demonstrate the efficacy of the tissue construct in vivo. Interestingly, the histology of the postimplanted tissue construct revealed the distribution of chicken wire net-like fat cells within the vicinity of the construct. The efficacy of cell transplantation via the scaffold was traced using fluorescent in situ hybridization by labeling the Y chromosome. Thus, the ceramic-based construct may be a good option for reconstruction therapies. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Issue Number 6
Page Count 11
Starting Page 1427
Ending Page 1437