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Author Pillai, Malini S. ♦ Shivakumar, K.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Molecular and Cellular Biochemistry
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health ♦ Diseases
Subject Domain (in MeSH) Cardiovascular Diseases ♦ Diseases
Subject Keyword Cardiology
Abstract Genistein, a soy isoflavone, is reported to exert significant beneficial action in several human disorders, which has generated immense interest in the mechanisms underlying its effects on diverse cellular processes. It has anti-proliferative action on many cell types, an effect generally attributed to tyrosine kinase inhibition. In this study, genistein was found to cause total inhibition of [(3)H]-thymidine incorporation into DNA and a modest reduction in [(3)H]-proline incorporation into protein in primary cultures of cardiac fibroblasts. The decrease in [(3)H]-thymidine incorporation was not associated with a decrease in cell proliferation but correlated exactly with low intracellular levels of [(3)H]-thymidine. Genistein dramatically reduced [(3)H]-thymidine but not [(3)H]-proline uptake by these cells in which the equilibrative nucleoside transporter may be the major route of nucleoside uptake. The effect was irreversible and was demonstrable in pulmonary fibroblasts as well. The findings suggest that nucleoside uptake mechanisms may be a novel target of genistein action in cardiac fibroblasts and point to serious limitations in using genistein to assess the role of tyrosine kinase in cell proliferation by the standard technique of [(3)H]-thymidine incorporation.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Journal MOLECULAR AND CELLULAR BIOCHEMISTRY
Volume Number 332
Issue Number 40910
Page Count 5
Starting Page 121
Ending Page 125