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Author Geetha, C. S. ♦ Sabareeswaran, A. ♦ Mohanan, P. V.
Source Sree Chitra Tirunal Institute for Medical Sciences & Technology
Content type Text
Publisher Toxicology Mechanisms and Methods
File Format PDF
Language English
Subject Domain (in DDC) Technology ♦ Medicine & health
Subject Domain (in MeSH) Inorganic Chemicals ♦ Biomedical and Dental Materials ♦ Chemicals and Drugs ♦ Investigative Techniques ♦ Analytical, Diagnostic and Therapeutic Techniques and Equipment ♦ Technology, Industry, and Agriculture ♦ Technology and Food and Beverages
Subject Keyword Biological Evaluation
Abstract The titanium nitride coated titanium is a material intended for the fabrication of left ventricular assist device. As per International standards, a material is subjected to pre-clinical evaluation before fabrication of a device. Toxicity/biocompatibility studies such as acute systemic toxicity, intracutaneous irritation, in vitro haemolysis and implantation in muscle were studied as per international standards for the titanium nitride coated titanium. Acute systemic toxicity was studied in mice using physiological saline and cotton seed oil extracts of the material. Intracutaneous irritation was done by injecting the extracts of the test material and control intradermally into rabbits. Grading of erythema and oedema of test and control animals were recorded at 24, 48 and 72 h. In vitro haemolysis was carried out with material and extract of the material with rabbit blood. The muscle implantation was carried out in nine anesthetized rabbits. The implanted animals were sacrificed at the end of 1, 4 and 12 weeks, the tissue with the implanted materials were collected and subjected to histopathological analysis. The results of the study did not show any significant irritation or systemic toxicity with physiological saline and cotton seed oil extracts of the material. The percentage of hemolysis induced by the material and extract was under acceptable range. Results of the histopathological evaluation suggest that the test material did not produce any cellular changes. Hence the present study concluded that the test material is non-toxic, non-irritant, non-haemolytic and biocompatible.
Education Level UG and PG
Learning Resource Type Article
Educational Framework Medical Council of India (MCI)
Volume Number 22
Issue Number 2
Page Count 7
Starting Page 144
Ending Page 150